Does anyone have a protocol for fecal DNA extractions without using a kit?

09 October 2021 1 4K Report

I am planning on using the QIAamp PowerFecal Pro DNA Kit on my samples, however, I am looking into alternative methodologies on effective ways to potentially extract DNA using lab resources when kits are not accessible. I want to look at microbial gut bacteria in the 16S gene. Thanks

Abhijeet Singh

You can always go for phenol-choloroform method. But kits are designed for a fast and easier/safer extraction process, which might not be the same for you if you use the traditional kit-less methods.

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