Hello everyone. I hope someone could give me a suggestion and tips&tricks.
I have been trying to detect p-eNOS on HUVECs cell line using WB and got nothing (literally my visualisation was clear) for several times. My antibody is p-eNOS (Ser1177) Rabbit polyclonal antibody from BEYOTIME and when the assay I did 1:1000 dilution.
The WB protocol I did was common. Do I need to add something (when the cell culture or lysis process) to induce p-eNOS expression?
Thank you!