I am trying to test for Jurkat activation following stimulation with peptide-pulsed APCs. I am looking for a flow-based assay. Are there jurkat markers other than CD69 that are up/downregulated upon TCR activation?
Hi there are a lot of other markers associated with the activation of T cells. CD25, CD38, and HLA-DR for example. Furthermore an intracellular staining for IFNg for example may also help.
Good luck!
Dear Donna,
you could try the following markers for your experiment.
There are some T cell activation markers that are upregulated (even though many have originally been defined by agonistic antibody mediated stimulation). Still the following markers might be worth a try:
CD2, (cy)CD3, CD5, CD28, CD44
Some of those markers might need sustained activation via peptide pulsed APC to be strongly upregulated, but I am sure you can resolve that doing a time course. In addition, you might wanna monitor downregulation of CD62L and/or cytokine production as Christian already indicated.
For more information you might wanna consider:
http://www.nature.com/leu/journal/v21/n2/fig_tab/2404486t1.html
Good luck with your experiment.
Michael
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