Hello everyone, I am trying to depolarize neuron cells using two different concentrations of KCl to ensure their osmolarity is the same. I measured the following:
The live imaging solution serves as the control. I kept my cells in it to take the first set of measurements as a control. This solution has KCl at 2.9 mM, and its osmolarity is 410 mOsm.
I used the same live imaging solution, but I increased the KCl concentration to 55 mM by dissolving a specific amount of grams of KCl in 10 ml of the live imaging solution. The osmolarity for this is 567 mOsm.
I also prepared a stock solution with 100 mM KCl, and its osmolarity is 501 mOsm. I dissolved a specific amount of grams of KCl in PBS to make a 100 mM KCl solution. From this solution, I diluted to make a 55 mM KCl solution in 10 ml of live imaging solution. I took 5.5 ml from the stock solution and completed the volume by adding 4.5 ml of live imaging solution. The osmolarity for this is 413 mOsm.
My question is, which one should I use to not affect the osmolarity? The compositions of PBS and live cell imaging solution are attached.
I am just keep everything same I just want study the effect of KCL on my cells
thank you
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