13 Questions 9 Answers 0 Followers
Questions related from Ryan ..
Dear All, I found a paper related to my work. I will do the same thing except I will change the reagent they use. For example, instead of using reagent X, I will use reagent Y. They use gold...
26 June 2024 5,839 0 View
Hello everyone, My question for sure is silly, but I have been arguing with my labmate about this. If our plasmid concentration is 0.776 µg/µL and he wants to make a final concentration for...
11 June 2024 9,974 5 View
Hello, I have 2 different questions: I am trying to extract mitochondria from mammalian cells and then activate the Electron Transport Chain (ETC) to study protons release. A) What kit do you...
12 December 2023 1,200 0 View
is this contamination post transfection using DreamFect Gold ? the transfection method is lipid based method picture is attached i am concern about the one in the yellow box
03 November 2023 2,547 4 View
Hello everyone, I am trying to depolarize neuron cells using two different concentrations of KCl to ensure their osmolarity is the same. I measured the following: The live imaging solution serves...
01 October 2023 3,732 0 View
the protocol said (Add 1-5 µl containing 1 pg-100 ng of plasmid DNA to the cell mixture) My plasmid concentration is 114 ng/µl The cell mixture ( competent cells ) is 50 microliter I used M*V =...
06 February 2022 6,030 3 View
How can I transfer transfect Culture of hippocampal neurons with GFP? the more I read I get confused correct me if I am wrong according to my understanding. 1) the neuron need to extract 2)...
01 January 1970 2,021 3 View
Hi my plasmid has GFP into as gene of interest I did transfect cell ( cell line from mouse) using lipofectamine 3000. I did observe the GFP under a microscope but I could not localize where is it?...
01 January 1970 4,043 3 View
01 January 1970 2,574 5 View
Hello, I am working with mouse cells, and I need to localize my protein. My protein should bind to voltage-gated potassium channel beta subunits. Can you please help me from your experiences?...
01 January 1970 2,011 1 View
01 January 1970 1,742 0 View
01 January 1970 2,809 0 View
Hello, I am interested in measuring voltage changes in my cells under high and low potassium concentrations. My cells express GFP, and after taking images under low or high potassium conditions,...
01 January 1970 1,628 0 View
