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Questions related from Ryan ..
Dear All, I found a paper related to my work. I will do the same thing except I will change the reagent they use. For example, instead of using reagent X, I will use reagent Y. They use gold...
26 June 2024 5,827 0 View
Hello everyone, My question for sure is silly, but I have been arguing with my labmate about this. If our plasmid concentration is 0.776 µg/µL and he wants to make a final concentration for...
11 June 2024 9,969 5 View
Hello, I have 2 different questions: I am trying to extract mitochondria from mammalian cells and then activate the Electron Transport Chain (ETC) to study protons release. A) What kit do you...
12 December 2023 1,192 0 View
is this contamination post transfection using DreamFect Gold ? the transfection method is lipid based method picture is attached i am concern about the one in the yellow box
03 November 2023 2,540 4 View
Hello everyone, I am trying to depolarize neuron cells using two different concentrations of KCl to ensure their osmolarity is the same. I measured the following: The live imaging solution serves...
01 October 2023 3,727 0 View
the protocol said (Add 1-5 µl containing 1 pg-100 ng of plasmid DNA to the cell mixture) My plasmid concentration is 114 ng/µl The cell mixture ( competent cells ) is 50 microliter I used M*V =...
06 February 2022 6,024 3 View
How can I transfer transfect Culture of hippocampal neurons with GFP? the more I read I get confused correct me if I am wrong according to my understanding. 1) the neuron need to extract 2)...
01 January 1970 2,016 3 View
Hi my plasmid has GFP into as gene of interest I did transfect cell ( cell line from mouse) using lipofectamine 3000. I did observe the GFP under a microscope but I could not localize where is it?...
01 January 1970 4,037 3 View
01 January 1970 2,564 5 View
Hello, I am working with mouse cells, and I need to localize my protein. My protein should bind to voltage-gated potassium channel beta subunits. Can you please help me from your experiences?...
01 January 1970 2,006 1 View
01 January 1970 1,737 0 View
01 January 1970 2,804 0 View
