Organic solvent for trehalose?

I am looking for an organic solvent for trehalose. DMSO works, methanol works, like ethanol, but both are not suited for the rest of my process. Any ideas or exprience here?

04 August 2020 6,984 3 View

Elution of an antibody on a peptide immobilized column did not work?

I am very disappointed. I coupled a peptide with an N-terminal cysteine on a sulfolink column. HPLC shows that the peptide immobilization was almost complete. After cyclisation of the serum the...

25 May 2020 1,265 8 View

Hi! Can anyone show me how to calculate the total anthocyanins content using the OD at 545, 535 and 515 nm?

27 January 2017 7,419 3 View

Would you suggest any software executing ANN (MLP type) where connections between neurons (in consecutive layers) can be separately defined by user?

10 October 2016 3,464 7 View

What are the specific possible biases involved in having one coder in a qualitative analysis?

27 June 2016 998 4 View

Is there a reference article documenting the preferred imaging modes in oncology?

I would like citing a reference in a manuscript about the most used imaging modalities in oncology. Did somebody could provide me with a pointer?

17 August 2015 5,791 3 View

What is a clear definition for radiomics?

I am looking for a clear definition of "Radiomic". According to different papers from: Kuo, 2007; Lambin. 2012; aerts. 2014; singanamalli, 2014 and some others, I am not sure if the concept of...

15 February 2015 6,726 8 View

How can I compute the limit of agreement with Bland Altman featuring heteroscedasticity?

I am analysing repeated measurement of ratio-scaled data. For doing that I rely on Bland Altman analysis. The common case where Limit of Agreement is horizontal can be adressed by most of the...

20 February 2014 4,709 2 View

How to manage Bland Altman analysis with more than one couple of measurements?

I consider three readers circularly involved in Bland-Altman 2-by-2; threfore I obtained 3 Bland-Altman analysis with corresponding Limit of Agreement (and Confidence Interval). In order to get...

16 January 2014 9,164 6 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View