Many enzymes require coenzymes (NADH, NADPH, CoA, TPP, etc) to catalyze biochemical reactions, such as redox reactions. Does an Enzyme bind to substrate and coenzyme at the same time or bind to one after another ? Is the binding of substrate to an enzyme independent of binding of coenzyme? Is there any specific order in which substrate and coenzyme bind to enzymes?
Hello Yijie! There are several types of kinetic mechanisms for substrate binding and product release for bisubstrate enzymes.
Steady-state random; rapid equilibrium, steady-state ordered (SSO), equilibrium ordered, Theorell Chance and Ping pong mechanism. These types of mechanisms can be determined through different ways: (1) initial velocity experiments (varying substrate 1 concentration, holding the other one constant - and vice versa); (2) substrate and product inhibition experiments; and (3) kinetic isotope effects (direct comparison of initial rates; equilibrium perturbation and internal competition).
There are good discussions of this topic from book chapters by (1) Cook and Cleland, (2) Silverman, (3) Jencks and (4) Frey.
Hi there,
There is no golden rule concerning the scheme of binding/release of substrates/products.
I think the type of the chemical enzymatic reaction and its mechanics, determine who is the first and second in the enzymatic reaction ...
Hello Yijie! There are several types of kinetic mechanisms for substrate binding and product release for bisubstrate enzymes.
Steady-state random; rapid equilibrium, steady-state ordered (SSO), equilibrium ordered, Theorell Chance and Ping pong mechanism. These types of mechanisms can be determined through different ways: (1) initial velocity experiments (varying substrate 1 concentration, holding the other one constant - and vice versa); (2) substrate and product inhibition experiments; and (3) kinetic isotope effects (direct comparison of initial rates; equilibrium perturbation and internal competition).
There are good discussions of this topic from book chapters by (1) Cook and Cleland, (2) Silverman, (3) Jencks and (4) Frey.
Archie C. Reyes is right, I'd just add Bisswanger 2017 (ISBN 978-3-5273-4251-8) to the list, which is IMHO particularly readable, yet thorough.
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