Any good ideas to study and grow the unculturble bacteria?

08 September 2016 385 3 View

Why there are often false positive bacterial colonies on antibiotic plates after DNA transformation?

During DNA transformation experiments, we sometimes see a few false positive transformants ---- bacterial colonies that carry no any insert plasmids or DNA fragments but can still grow on...

07 August 2016 3,664 5 View

Suggestion to increase the transformation efficiency for non-model bacteria?

04 May 2016 2,142 2 View

What is the limiting factor in CO2 reduction by electricity?

There are some reports on reducing CO2 into fuel chemicals but the efficiency is quite low. What do you think is the limiting factor of CO2 reduction? We have to identify the rate-limiting factor...

01 February 2016 1,086 6 View

How can I express and purify oxygen-sensitive enzymes?

08 September 2015 4,760 5 View

How do you screen thermo-stable enzymes from mutant library in a high throughput way?

06 July 2015 9,838 7 View

What do you think the potential of cell-free systems in biotechnology?

03 April 2015 244 6 View

Do you know any good academic conferences for bioenergy, biofuels and biotechnology?

03 April 2015 4,683 0 View

Is an enzyme complex encoded by a single gene, an operon or several independent genes?

02 March 2015 9,769 8 View

What is the main difference in the electron transport chain systems between aerobic and anaerobic bacteria?

Both aerobic and anaerobic bacteria need to have ETC to synthesize ATP. While aerobic bacteria use oxygen as the terminal electron acceptor, anaerobic bacteria use other substrates as the electron...

02 March 2015 2,425 13 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View