Which is the best marker to quantify embryonic microglia in hypothalamus by flow cytometry?

I need to quantify embryonic microglia (E17) by flow cytometry. Anyone has experience in this? Thank you!

03 March 2020 8,841 3 View

Hello, can someone please tell me how to make this kind of graphics in cst studio suite ?

I am designing micrsotrip antennas to capture wireless energy and I would like to see the power density vs. frequency of the microstrip antennas attached to the images.

22 January 2020 1,448 3 View

Propidium iodide for gel electrophoresis?

Good afternoon, Propidium iodide (PI) is a dye use to stain nucleic acid in flow cytometric analyses, particularly in cell cycle analyses. Has anyone dared to try staining a DNA electrophoresis...

29 April 2019 7,100 3 View

Which is the most efficient method to determine the tensile strength of Rice Husks/Epoxy Lamina by FEA?

03 March 2017 775 3 View

Can you give me advise on TNF-alpha ELISA kits?

I need a to perform an immunoassay of TNF-alpha on human serum. I am looking for advise on brands that can offer a good (the best if possible) balance price/quality/simplicity. If you could share...

06 December 2015 9,618 2 View

Which is the best way to purify TiCl4?

I used to use TiCl4 in a Dieckmann condensation and yields were always high, however some days ago, I repeated the experiment twice and product is not obtained; I detected starting material and...

25 January 2015 4,044 3 View

Which is the best way to purify TiCl4?

I used to use TiCl4 in a Dieckmann condensation and yields were always high, however some days ago, I repeated the experiment twice and product is not obtained; I detected starting material and...

31 December 2014 3,202 3 View

Is it possible to obtain a tertiary amine from the corresponding ammonium iodide salt?

02 March 2014 6,602 8 View

Can someone give me information of an Unsignalized U-turns?

Can someone give me information of a capacity, geometry and safety of an Unsignalized U-turns. Thanks

01 January 1970 7,670 6 View

How to use ICT for social integration?

IMPORTANT: Best practices and answers were published in the ASIDE project reports [ISBN], EPALE and in the project website. http://aside.inbie.pl/ Modern adult education is one in which the...

01 January 1970 3,254 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View