I am planning to perform an ORAC assay. I have read in several papers that authors use a black plate, and others don't. Is it necessary? What kind of plate is the adequate for this kind of assay? I am testing a plant lysate...
I want to evaluate the polarization to M2 phenotype in Raw Cells, I co-cultured the cell with LPS and my drug to see the effect of the drug to inhibit the proinflammatory effect of LPS, but I have...
05 May 2024 8,525 0 View
Hi, I want to evaluate stress oxidative markers in macrophages using western blot. I want to know an easy protocol to isolate macrophages from PBMCs, I already know a protocol to isolate them...
24 August 2023 1,488 2 View
I want to study the expression of some proteins related to oxidative stress with western blot. I wonder what microglia cell line is the best for it.
20 August 2023 456 3 View
When I'm running SDS-PAGE 12%, my sample moves to the other well (it's formed as a small curve with the following well) , even if I put it slowly,carefully, 15ul per well and I'm using a 1mm...
02 July 2023 3,344 3 View
I'm using a molecular weight size marker (cat #161-0373), but I don't have all the bands and lately the last band I get was not straight. I run in a 12% gel, I use 80v for the stacking and 200v...
02 July 2023 1,069 0 View
Hi, I want to evaluate oxidative stress in a neuroblastoma cell line, I know about SOD1, but I think it's not enough, so I was investigating and I found 4-hydroxynonenal, I have this in my lab...
27 June 2023 9,084 3 View
Hi! I am trying to purchase these suspension cells but I can not find a company that provides them...Am I making something wrong? Thnx!!
27 March 2023 8,302 1 View
Hi all! I have some 16S sequences amplified with the universal primers 341F: CCTACGGGNGGCWGCAG & 805R: GACTACHVGGGTATCTAATCC. I have checked the sequences and primers are present in all...
22 January 2023 5,368 3 View
Hi all! I have paired end reads of the V3-V4 region of the 16s. PCR amplification and sequencing was performed at macrogen facilities, in Korea. Sequencing was performe with Illumina MiSeq. I...
11 January 2023 3,721 1 View
Hi, there Does anyone has experience with (MPBIO) FastDNA spin Kit for soil DNA extraction intrinsic contamination? Salter et al. 2014 test it, and it had several intrinsic microbial...
20 November 2022 7,185 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View