Having 2 different substrates under the same deposit conditions (TiN coating) why it is possible to obtain different colours in the 2 dif substrates?

Coating is made by same deposit conditions. substrates are HSS Drill bit and Shim SS 300 series. On the drill bit we are getting some golden color, however on the shim we got no color it is like...

08 February 2021 9,383 7 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Why does the 2D peak intensity in a graphene Raman spectrumdecrease with the number of layers?

If we compare a Raman spectrum of monolayer graphene and bilayer or fewlayer graphene, we will see that the 2D peak intensity decreases (relative to the G peak intensity) for the later cases. I...

24 July 2020 907 3 View

Which is the best marker to quantify embryonic microglia in hypothalamus by flow cytometry?

I need to quantify embryonic microglia (E17) by flow cytometry. Anyone has experience in this? Thank you!

03 March 2020 8,841 3 View

Cas9 in vitro clevage, no bands or bigger bands?

12 February 2020 8,781 1 View

Hello, can someone please tell me how to make this kind of graphics in cst studio suite ?

I am designing micrsotrip antennas to capture wireless energy and I would like to see the power density vs. frequency of the microstrip antennas attached to the images.

22 January 2020 1,448 3 View

Which are the principles of AEX (Anion Exchange Chromatography)?

I have been trying to find out how this method works, but the papers I find are related to the application of this technique and not to the basic principle of it. Any helpful link will also help...

23 October 2019 9,470 2 View

Propidium iodide for gel electrophoresis?

Good afternoon, Propidium iodide (PI) is a dye use to stain nucleic acid in flow cytometric analyses, particularly in cell cycle analyses. Has anyone dared to try staining a DNA electrophoresis...

29 April 2019 7,100 3 View

With SOLID185 microplane damage model. How do I solve my error in Ansys??

In the last weeks I have worked the damage model of the element SOLID65, but it has not allowed any results, so I decided to work with the element SOLID185, with the recommendations of the...

07 March 2019 5,662 5 View

ORAC black plate?

I am planning to perform an ORAC assay. I have read in several papers that authors use a black plate, and others don't. Is it necessary? What kind of plate is the adequate for this kind of assay?...

03 April 2018 6,314 0 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

Calculating the CFU?

i am constructing research for the antibacterial activity of the medication and I am testing it by %RCC, so my Q after I did 10 serial dilutions for the bacterial suspension can we do plating for...

04 February 2021 5,886 1 View

Where to buy optically clear, low viscosity, refractive index 1.5-1.4 Silicone Oil ?

For research purposes, it is required to have optically clear, low viscosity silicone oil with a refractive index of 1.5-1.4. It is going to be used in optoelectronic research. Online purchase is...

01 February 2021 9,160 3 View

Lupasol G100 from BASF -- where to buy?

Hello everyone. I am looking to purchase Lupasol G100 (essentially ~5kDa bPEI) for creation of carbon dots for in planta transient gene expression. Thus far, I have only been able to find a...

20 January 2021 7,683 1 View

How to de-clump HEK293 suspension cells?

I have a problem with clumping of HEK293 suspension cells, and this clumping problem decreases the cell viability. I will transfect the cells if I were to reach a favorable cell count. What can I...

17 January 2021 354 4 View