Naomi Hitefield

4 Questions 2 Answers 0 Followers

Questions related from Naomi Hitefield

Mitochondrial Membrane Uncoupler Suggestions in 96-Well Assay?

We have a 96-well assay we use to measure mitochondrial membrane potential. A 200 uL volume of cell resuspension is in each well. We want to add a membrane uncoupler, like FCCP, to uncouple the...

01 January 2019 5,510 0 View

JC-1 and JC-10: Experience with Flakiness of Dye and Substituting JC-1 with JC-10?

Hi, fellow researchers. We currently use JC-1 dye in the lab, but we have problems with the dye (2 uM) flaking out of aqueous buffers. I found JC-10 online, and companies state this dye is...

06 June 2018 9,623 0 View

Has anyone received negative RFU values for one of your replicates in qPCR?

We are running qPCR with SYBRGreen to amplify an inserted 12S ribosomal DNA region of a plasmid. When we ran this amplification for the first time (in duplicate), one replicate looked fine while...

04 April 2018 1,715 4 View

Optimal Cell Number for Nitrogen Cavitation of Cells?

Hi! We are isolating mitochondria from platelets using nitrogen cavitation via a Parr bomb apparatus. Has anyone had experience isolating mitochondria from blood or other culture cells using...

04 April 2018 9,174 1 View