Is it time for serious action on climate change?

30 November 2020 9,105 5 View

Why antenna reflection coefficient value changes when the frequency sweep range is changed?

Many times, what I observed is that the s11 of the designed antenna is changed when I change the frequency sweep in hfss. I can't understand, that why does the antenna response change. Please...

30 September 2020 4,129 2 View

Could someone explain the common cause of mismatching in reflection coefficient at higher frequencies in the measured result of antenna?

Many times the measured results of antenna matches with simulated but sometimes measured results do not match with simulated and sometimes measured results do not match at higher frequencies. Plz...

07 September 2020 3,550 14 View

DNA and RNA extraction from tissue sample ?

Is there a simple extraction process for both DNA of a bacteria and RNA of a virus from infected tissue sample without losing either DNA or RNA from the sample ???

08 June 2020 2,245 4 View

Which stain is used to see the deposition of oil or oil glands in anatomy section of leaf(transverse section) in cymbopogon?

Cymbopogon is an aromatic grass which produces essential oils as a secondary metabolite so to see the deposition of oil in leaves transverse section of intra and inter species can you suggest me...

28 August 2019 9,357 2 View

How to clone of Github NetSim project?

18 June 2019 1,289 2 View

How do I encrypt NetSim Payload encryption through MATLAB?

Instead of using the default encryption algorithms in NetSim, I want to use MATLAB to encrypt the packet payload of Netsim. This needs to be done for everypacket generated in NetSim. How can I do...

16 May 2019 1,886 1 View

What should be the strategy to increase pulses production in India?

How we can transform India from a pulse deficit country to pulse surplus state.

10 April 2019 4,639 3 View

How can poverty be reduced in future with the help of Agri-entrepreneurs?

Agripreneurship development is a key tool for poverty reduction. It has been proven effective for stimulating employment and economic growth in developing countries in Asia and Africa....

12 March 2019 6,261 3 View

What is the appropriate protocol for generation of Dendritic cells from mouse bone marrow..should it be GM-CSF+IL-4 or only GM-CSF?

Hi everyone, I am trying to generate Dendritic cells from mouse bone marrow where I am culturing the bone marrow cells with GM-CSF for 6 days and measuring the percent DCs generated on 6th day by...

07 March 2019 4,561 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View