DNA and RNA extraction from tissue sample ?

Is there a simple extraction process for both DNA of a bacteria and RNA of a virus from infected tissue sample without losing either DNA or RNA from the sample ???

08 June 2020 2,245 4 View

Which is the best solvent/ solution for the storing intestinal biopsy sample at -80 deg Celsius? and for how long can I store it ?

I need the tissues for the isolation of leukocytes for drug metabolite analysis. How long will they be stable at - 80 deg Celsius.

30 September 2016 3,751 1 View

I need a protocol for the isolation of leukocytes from intestinal mucosal tissue and which solvent to be used to store the tissues before isolation?

19 September 2016 8,924 3 View

Which is an appropriate column to be used for the analysis of nucleotides like thioguanine mono/di/tri phosphates for LC-MS ?

If possible kindly provide me a reference for the same.

19 September 2016 1,633 4 View

Validation of overexpression by microinjection, any ideas?

Hello everyone, I am overexpressing a gene in Zebrafishes, by using some neural crest cell promoter, through microinjection. I do see some phenotype, but so far, I have seen it in a transgenic...

14 March 2016 2,396 8 View

I have to identify fungal isolates from soil. Which primer pair is better, ITS1/ITS4 or ITS4/ITS5?

They are likely ascomycetes

09 March 2016 9,183 1 View

Can anyone help with molecular dynamics -PCA analysis in gromacs?

I have performed molecular dynamics simulation for 50ns in Gromacs. The protein is stabilized after 20ns. I did PCA analysis for last 30,20,10 and 5 ns. Is there any criteria to select the time...

05 June 2014 6,583 2 View

How to determine measurement uncertainty of a mass balance?

Hi everyone, I work at a factory plant where they use a mass balance. The problem is that there is an huge difference between the input and output of one element. There are several people working...

10 February 2021 5,764 3 View

I have a problem with sensible heat flux (H) iteration process in Sebal model?

In each iteration, I get the same value of H for different values of aerodynamic resistance and the temperature difference between two heights. Do the results make sense? Is it necessary to adjust...

07 February 2021 8,953 2 View

What are the advantages and limitations of online teaching mode of HEIs; and how we can make a balance between them?

With the increase in use of online modalities during the current situation, it is necessary to assess the advantages and limitations with regards to teaching and learning from various...

04 February 2021 4,558 8 View

Data balancing before test/train split or only training data balancing. which is correct?

While reviewing technical articles, I encounter some papers where authors have used data balancing before splitting test and train (on full data), and some only balanced training data and let the...

04 February 2021 1,446 0 View

Can anyone provide me with the scoring method and the questionnaire of Work life balance scale by Hayman (2005)?

Greeting! For my MA dissertation, I am thinking of taking Work life balance scale by Hayman. However, I can't find the scoring procedure; also confused about the actual rating taken. If anyone...

23 January 2021 4,074 7 View

How to propose constitutive response functions during continuum modeling?

To develop constitutive models in continuum mechanics, one must consider kinematics, balance of momentum, balance of energy, and free-energy imbalance. Next, one must propose constitutive response...

02 January 2021 9,692 4 View

Do you know what happen in reaction between Ni2SO4.6H2O and DMG (dimethylgolyoxin)?

I want to use DMG as a reagent for detecting Ni in a solution. in their reaction they form a complex together which color is pink. I attached a picture of the reaction. my problem is I can`t find...

01 January 2021 7,200 3 View

What is the impact of Pandemic on the higher education faculty?

Work life Balance, Stress, Anxiety, Effective teaching

28 December 2020 6,201 3 View

Panel data estimator with small sample size, which to use?

Dear all, I have a strongly balanced panel of 22 countries for 13 years. I am interested in finding the best estimator to run a regression (with max 7-8 regressors) in STATA. I am also taking...

09 December 2020 2,337 3 View

Tips for students on how to take care of a work-life balance during a covid19 pandemic ?

Students asked me for advice on how to deal with work-life balance during a lockdown. Some of them work and study from home. Do you have any helpful suggestions? I found an article that suggests...

29 October 2020 5,994 2 View