Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Contamination when culturing primary uterine endometrial cells?

Dear everyone I'm working on the endometrium of the uterus. I need to extract endometrial cells from the endometrial tissue. Usually, I will separate the tissue into 2,3 mm pieces and incubated in...

25 November 2020 7,428 3 View

What are the morphological changes of PC3 cells during autophagy and/or apoptosis?

I was performing wound healing assay seeding 300,000cells/well in 6 well plates with 2mL RPMI medium. At 0hr after intervention, the cells looked like the first picture. At 24hr after...

18 November 2020 9,671 2 View

Does PROCESS v3.5 by Andrew F. Hayes with SPSS Statistics 27 on MacOS 10.15.7 work?

After installing the PROCESS v3.5 tool, I ran a mediation model 4 with three variables (N = 125). The warnings below showed up on the output window, and the status at the bottom shows, "Running...

15 November 2020 3,930 14 View

How do you deal with massless particles in SR, since they can't have four vectors?

Suppose a photon is observed to have some three velocity v in an inertial frame O, another inertial frame O' is moving away from O with another three velocity u. Is there a general way to get the...

10 October 2020 5,132 3 View

What are the peaks at 14.3 and 28.4 in LDH's XRD pattern?

Hi! I'm synthesizing MgAl-NO3-, MgAl-Cl-, MgAl-CO32-, etc. by coprecipitation method using metal nitrate salts, NaOH, and anion source coming from sodium salts. Besides those characteristic peaks...

21 September 2020 3,402 3 View

The correctness of synthetic data and corresponding label generating from smote or adasyn ?

Dataset imbalance is a major problem usually encounter in machine learning practice. Some technique such as SMOTE or ADASYN generate addition data (synthetic data) to avoid imbalance problem....

06 September 2020 3,337 3 View

PC-3 cells: what does a neuron like/threadlike morphology indicate about the cell's condition?

I was performing wound healing assays with PC-3 cells (cultured in RPMI+10%FBS; 500,000 cells/well in 6 well plate). I seeded them for 24 hours, then applied the scratch with a 100uL pipette tip....

03 September 2020 7,664 4 View

Do you have any references on "Process to develop Child centered Social accountability"?

Dear all , I am working as the consultant for Save Children International to develop and pilot a process named " Child Centered Social accountability" in Vietnam. Does anyone know where I can get...

31 August 2020 6,297 5 View

How to build up a cell model for endometrium receptivity?

I want to conduct some research on the topic of endometrium receptivity, but I only have HEC1A cell line available. Can you please share with me the experience in using which cell line for this...

26 August 2020 2,381 1 View

No title

02 March 2021 324 2 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

How to select from various invitations from Scientific Boards in conferences and proceedings?

How to select from various invitations from Scientific Boards in conferences and proceedings? How to spot the ones with high impact and genuine? Can we spot valuable events?

02 February 2021 8,187 1 View

What is the most appropriate design (statistic test) to assess the toxicity of 2 different compounds at 6 different concentrations and 2 conditions?

10 January 2021 8,077 3 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

Is it easy to collect a conference certificate?

share your experience please.

01 January 2021 5,084 4 View

Which test to use check normality for count data if I have 3 treatments and one dependent variable?

I am working on a experimental design. I have three treatments(three temperatures) . I have 18 fish under each sample and after a desired time I will check if there is any variation in their...

01 January 2021 9,167 3 View