Nuclear extraction of mouse liver?

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What is the best way to extract triglyceride from cells?

Hi all, I would like extract TG from mouse primary hepatocytes and measure with enzyme-cased color absorbance. So, What is the best way to extract TG from the cells?

10 November 2018 6,436 2 View

Primary hepatocyte siRNA transfection?

Dear all, Currently, I am trying to transfect siRNA to mouse primary hepatocytes. I am wondering if anybody has some detailed protocol about the transfection. I am using lipofectamine RNAiMAX as...

03 April 2018 7,772 4 View

Which lobe of the mouse liver should be collected for real-time PCR?

Hi everyone, I am a bit curious about the protocol of collecting the mouse liver for real-time PCR. As I would like to check the expression of the gene under two different conditions. However,...

09 October 2017 8,237 0 View

Too high amplification efficiency?

Hi all, I am conducting a real time PCR for my gene of interest. For the housekeeping gene, I could produce a really good standard curve (R square:0.9 with approximately 105% efficiency and...

08 September 2017 3,585 0 View

Using cAMP+Dexamethasone to mimic the signaling of Glucagon

Hello all, I hope all of you are doing fine. As we know well that Glucagon and Glucocorticoid activate hepatic gluconeogenesis via cAMP. I found a paper using cAMP/dex to treat to cultured cell...

07 August 2017 4,044 0 View

Standard curve for qPCR

I am conducting the qPCR to check the gene expression. After reverse-transcribing the RNA, I am going to perform the qPCR of cDNA. In my laboratory, to make a standard curve for qPCR, we have to...

05 June 2017 1,194 16 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View