Nuclear extraction of mouse liver?

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What is the best way to extract triglyceride from cells?

Hi all, I would like extract TG from mouse primary hepatocytes and measure with enzyme-cased color absorbance. So, What is the best way to extract TG from the cells?

10 November 2018 6,521 2 View

Primary hepatocyte siRNA transfection?

Dear all, Currently, I am trying to transfect siRNA to mouse primary hepatocytes. I am wondering if anybody has some detailed protocol about the transfection. I am using lipofectamine RNAiMAX as...

03 April 2018 7,819 4 View

Which lobe of the mouse liver should be collected for real-time PCR?

Hi everyone, I am a bit curious about the protocol of collecting the mouse liver for real-time PCR. As I would like to check the expression of the gene under two different conditions. However,...

09 October 2017 8,343 0 View

Too high amplification efficiency?

Hi all, I am conducting a real time PCR for my gene of interest. For the housekeeping gene, I could produce a really good standard curve (R square:0.9 with approximately 105% efficiency and...

08 September 2017 3,631 0 View

Using cAMP+Dexamethasone to mimic the signaling of Glucagon

Hello all, I hope all of you are doing fine. As we know well that Glucagon and Glucocorticoid activate hepatic gluconeogenesis via cAMP. I found a paper using cAMP/dex to treat to cultured cell...

07 August 2017 4,091 0 View

Standard curve for qPCR

I am conducting the qPCR to check the gene expression. After reverse-transcribing the RNA, I am going to perform the qPCR of cDNA. In my laboratory, to make a standard curve for qPCR, we have to...

05 June 2017 1,294 16 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View