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Questions related from Jojo Redb
Hi all, I would like extract TG from mouse primary hepatocytes and measure with enzyme-cased color absorbance. So, What is the best way to extract TG from the cells?
11 November 2018 6,489 2 View
Dear all, Currently, I am trying to transfect siRNA to mouse primary hepatocytes. I am wondering if anybody has some detailed protocol about the transfection. I am using lipofectamine RNAiMAX as...
04 April 2018 7,802 4 View
Hi everyone, I am a bit curious about the protocol of collecting the mouse liver for real-time PCR. As I would like to check the expression of the gene under two different conditions. However,...
10 October 2017 8,315 0 View
Hello, I would like to inquire if anybody has the protocol for nuclear extraction of mouse liver. Thanks
10 October 2017 3,640 3 View
Hi all, I am conducting a real time PCR for my gene of interest. For the housekeeping gene, I could produce a really good standard curve (R square:0.9 with approximately 105% efficiency and...
09 September 2017 3,617 0 View
Hello all, I hope all of you are doing fine. As we know well that Glucagon and Glucocorticoid activate hepatic gluconeogenesis via cAMP. I found a paper using cAMP/dex to treat to cultured cell...
08 August 2017 4,078 0 View
I am conducting the qPCR to check the gene expression. After reverse-transcribing the RNA, I am going to perform the qPCR of cDNA. In my laboratory, to make a standard curve for qPCR, we have to...
06 June 2017 1,266 16 View
