06 February 2017 1 4K Report

Any of you have experience using NSG mice? Do you know if these mice express microglia cells in brain?

More Andres Fernandez Gil's questions See All
¿Where to buy the Aspergillus oryzae NSAR1 strain?

Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...

11 July 2024 6,847 0 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View

What is the optim temperature for protein denaturation in supernatants??

Hello! I´m developing a sandwich ELISA. My assay recognizes protein denaturalized very well, but there isn´t recognition when it is in its native form. So maybe if I heat the sample I could detect...

18 April 2024 5,671 3 View

Does anyone have access to literature supporting the idea that there are differences in energy demand across different levels of a building?

I'm working on the theoretical framework of my thesis and I can't find literature to support the idea that at different floor heights, there may be differences in energy demand. If someone can...

25 March 2024 4,998 1 View

How can I improve my electrophoresis technique?

Hello. I'm making non-denaturing PAGE of DNA fragments for analysis of polymorphisms, but I'm getting wavy bands. If somebody can help me, I will be very greatful. First of all, here's my recipe...

15 March 2024 7,699 1 View

Problems isolating human neutrophils using Percoll - Erythrocytes not pelleting?

Hello all, I present here a problem to see if someone has encountered it and can provide some clarity. We are trying to isolate neutrophils from patient blood in tubes EDTA. We are following the...

10 March 2024 4,156 3 View

Is anybody interested in initiatives to curb food waste at the consumption stage?

"Creative and Sustainable Initiatives to Combat Food Waste at the Consumption Stage" This Special Issue on combatting food waste through responsible consumption aims to gather the latest results...

29 February 2024 3,822 6 View

Do my iPSCs have a normal morphology?

Hello everyone, I am starting to grow the SCTi003-A iPSC line for the first time and I am facing some drawbacks. Due to some problems with the first pass, I waited a bit longer than the 7-8 days...

22 February 2024 8,052 2 View

Transcriptomic data sets with Staurosporine?

Hi, Does anyone know about a public transcriptomic dataset (RNAseq, microarray) of Arabidopsis (or other plants) treated with Staurosporine? I checked in GEO or SRA, but I couldn't find any....

11 February 2024 8,621 0 View

Potassium Permanganate titration- more hydrogen peroxide detected than what is actually present in solution?

I have been performing titrations of a known concentration of hydrogen peroxide using 0.01N potassium permanganate and consistently requiring more potassium permanganate than what is estimated to...

07 February 2024 446 3 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close