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Questions related from Andres Fernandez Gil
I have infected iPSCs with a lentivirus and sorted GFP positive cells. But after just a few rounds I am finding cells without GFP in culture. Had anyone seen this? Maybe cells are segregating...
18 October 2023 1,583 1 View
We are trying to purify human iPSCs using lentiviral induced GFP expression. But is being really difficult to achive this. Cells are usually dying after sorting. Any experience on this? anyone...
26 September 2023 6,952 2 View
Does anybody knows of a good online desing tool for qPCR primers and probes? Roche universal prove desing tool is close to its ending. thanks a lot.
08 March 2021 3,882 3 View
We are performing experiments on glioma cells. After infection of a cell line (U251), we see that Calcium levels increas even using a mock virus. Any reference that could explain this? Maybe...
04 March 2019 7,965 1 View
Anyone knows if monocites proliferate when in culture with M-CSF, N2 and serum? I mean create a clonal expansion in vitro, not only diferenciate into macrophages? Bibliografy is some confusing,...
19 October 2018 3,268 3 View
we are performing intracraneal injection of in NOD SCID Gamma mice, and we are finding unusual problems with anesthesia, loosing 75% of mice during surgery or next following days. From last 8...
22 May 2017 9,444 2 View
I am considering using Thermo GeneJET Maxiprep kit, it´s an obvious question of price. Anyone has use this before? and specially using the plasmid preparation to produce lentiviruses? Thanks a lot!
23 April 2015 6,692 3 View
I am producing several lentiviruses for different purposes, but I am finding some amazing results: an old stock of certain virus can infect with no problem mouse and hume glioblastome cells (GL261...
16 December 2014 7,450 2 View
