Andres Fernandez Gil

5 Questions 4 Answers 0 Followers

Questions related from Andres Fernandez Gil

QPCR primer desing?

Does anybody knows of a good online desing tool for qPCR primers and probes? Roche universal prove desing tool is close to its ending. thanks a lot.

08 March 2021 3,874 3 View

Any data regarding Calcium levels in glioma cells cytoplasm after adenovirus infection?

We are performing experiments on glioma cells. After infection of a cell line (U251), we see that Calcium levels increas even using a mock virus. Any reference that could explain this? Maybe...

04 March 2019 7,931 1 View

Does anyone find problems anesthetizing NSG mice with ketamine/Xilacyne?

we are performing intracraneal injection of in NOD SCID Gamma mice, and we are finding unusual problems with anesthesia, loosing 75% of mice during surgery or next following days. From last 8...

22 May 2017 9,437 2 View

Does anyone have any feedback from using the Thermo GeneJET Maxiprep kit?

I am considering using Thermo GeneJET Maxiprep kit, it´s an obvious question of price. Anyone has use this before? and specially using the plasmid preparation to produce lentiviruses? Thanks a lot!

23 April 2015 6,684 3 View

Can the specificity of Lentiviruses change between different stocks?

I am producing several lentiviruses for different purposes, but I am finding some amazing results: an old stock of certain virus can infect with no problem mouse and hume glioblastome cells (GL261...

16 December 2014 7,443 2 View