Not able to do Digestion ??

24 February 2017 1 5K Report

Hello

Our lab has designed a fusion syntheic gene. we have ligated it to pCOLD 1 vector but after repeated attempts digest and release the insert we failed. do any one has any idea about this pCOLD vector sequence

Ruipeng Wang

Hi Mayank,

You can find the sequence from Addgene.

https://www.addgene.org/vector-database/2261/

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