I am working with mouse primary cortical neurons that are plated in a 96-well plate. I treat the cells with a ranging drug concentration for 24 h and then measure LDH using a kit to determine cytotoxicity. I also stain the cells with Propidium Iodide and DAPI and image the cells to obtain information about cell number and if they are alive/dead. I've been tasked with normalising the absorbance values against the cell number obtained from the staining to make sure that the result being obtained from the LDH is reliable and not due to different number of cells. Could you help me figure out what calculation/formula I need to use to normalise the absorbance values to cell number? Thank you.
Please look at the following below links which may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501254/pdf/nihms871020.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458191/pdf/nihms663675.pdf
https://hal.archives-ouvertes.fr/hal-01133372/document
Thanks
Hi there,
As you already wrote it you have to divide absorbance signal (of LDH activity) by the number of cells in the sample.
For actual formula for%cytotoxicity ..please find the Attached image
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