Non coding RNAs - FAQS.TIPS

More Mesut Karahan's questions See All

How to apply gravity load and vertical earthquake loads in dynamic implicit analysis?

I would like to ask how to apply gravity load. When I apply gravity load in the different step, and in the same step with earthquake load, I got different floor response spectrum results. Which...

08 November 2023 3,593 0 View

How to apply different damping ratios in vertical and horizontal directions in dynamic implicit analysis in ABAQUS?

I would like to ask how to apply different damping ratios in different direction. In modal dynamic analysis I can apply them with defining their own frequencies (1st mode for X direction, 3 mode...

08 November 2023 337 2 View

Can we use lipoproteins for COVID-19?

COVID-19 is a worldwide threat to humanity and spread fast. There is no effective vaccine yet, the combined antiviral therapies are performed and have a higher success rate. But we need additional...

05 July 2020 8,280 5 View

Inhaler antiviral therapy may be more effective than systemic antiviral therapy in Covid-19 patients?

... There is an urgent need for information on virus replication, immunity and infectivity in specific sites of the body. Here we report a detailed virological analysis of nine cases of COVID-19...

27 May 2020 9,812 4 View

Is macro-evolution natural selection or chaotic selection?

Discussion killed by prejudice, please do not reply!!!

08 May 2018 4,505 20 View

Do microorganisms use humans as a carrier?

Every member of the human population contains approximately 10131014 cells, 70% of which are cells of symbiotic bacteria predominantly localized in the gastrointestinal tract. Sometimes , I think...

18 April 2018 2,093 14 View

What do you think about cancer test, called CancerSEEK?

More than 2 years ago, Tomasetti and Vogelstein brought that question to prominence when they tried to sort out environmental versus inherited causes of cancer. They examined the extent to which...

21 January 2018 2,235 9 View

Why do we use "0"(zero) in physics ,math formulations? Is there "0" in universe?

Sometimes , we use term of "zero time" in a formulation but are we sure it is really "0" ? maybe it is 0,000......1 and is there a "zero" time(can we stop the time?), or sometimes, we say v=0 are...

18 January 2018 8,890 74 View

Is the speed of cosmic inflation equal to speed of time(Cosmic inflation is time)?

According to the theory of inflation, the Universe grew faster than light and I think speed of inflation is "speed of time" at the same time.We are convicted of this inflation(time) with gravity.

12 January 2018 3,073 15 View

Is manipulating gut microbiota hope for colorectal cancer therapy? Is "Chaotic Adaptation Theory(CAT)" correct?

Accumulating evidence demonstrates that intestinal bacteria influence oncogenesis, tumor progression, and response to therapy. Thus, selectively manipulating the gut microbiota may represent a...

29 October 2017 7,434 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Saleh Alkarim

Hello

You can try with one of these methods :

lncRNA immunoprecipitation, lncRNA pull-down, lncRNA northern blot analysis, lncRNA in situ hybridization, and lncRNA knockdown.

The link here will help you

Article Methods for the Study of Long Noncoding RNA in Cancer Cell Signaling

Good Luck

Pratibha Srivastava

Hi,

I hope you will find this article useful; illustration of a new technique R-Deep for the same.

https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30310-7

Good Luck.