Hello Folks,

I have been using the Neurite Tracer for measuring the lengths of the dendrites of B16F10 cells treated with some melanogenic compound. The actin filaments were stained with Phalloidin and nucleus with DAPI. I have followed a complete protocol as suggested by the developers (https://fournierlab.mcgill.ca/styled-6/NeuriteTracer.html). When I open the images for setting up the threshold, the processed image of nuclues was shown to be blank unlike neuronal images in which complete image is displayed. Additionally, the tracings of the counted dendrites have been shown but in the results no nuclear counts were measured.

Here I have attached image of the tracings, results and exception. Any suggestions would be highly appreciated.