No bands at lower molecular weights for eWAT ponceau on nitrocellulose membrane?

13 March 2024 0 10K Report

Hi everyone

I have been running some western blot samples on my eWAT. I run, transfer, block, primary, secondary, ECL and then image and ponceau after this. I was just wondering, when I ponceau my membranes, no bands after around 25kDa show up. I let the protein run until the 20kDa ladder band shows up. Is this normal?

Badges
Science topic

More James Smorenburg's questions See All

Is there an English Translation of the Carl Moller text: ZUR VERGLEICHENDEN ANATOMIE DER SILURIDEN?

I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...

10 August 2024 4,347 1 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

How to solve the question of ansys fluent?

Regarding the system coupling issue where the update of the solution component fails, the system coupling update for fluid flow (Fluent) triggered an exception: "Update Solution Failed."

30 June 2024 7,894 1 View

What is the problem with the request website?

I try to send papers to everyone who requests one and have a 93% rating. For over a year, I have received notices from ResearchGate that I have 18 open requests, including from a particular...

23 June 2024 5,844 1 View

Design a spectral filter?

How can I design a spectral filter by diffraction grating in Mamyshev oscillator? Is there any formula or article that I can refer to?

16 June 2024 1,231 1 View

Optimal conditions for lentiviral transduction of B-ALL cells (I'm using Nalm6 cells)?

Dear Esteemed Researchers, I am currently waist-deep into the literature to determine the optimal cell density and transduction volume for lentiviral transduction conducted in 24- and 48-well...

10 June 2024 4,167 0 View

Laser Capture Microdissection using Zeiss Palm microbeam instrument ..?

Dear Researchgate community. Is anyone doing laser capture microdissection using the Zeiss Palm microbeam instrument? If so, do you have an alternate (non-Zeiss) source for 500uL adhesive...

06 June 2024 5,117 2 View

Please, how can I calculate sample size from a population using G power?

I am working on a research and I need to determine what sample size to use for the study.

24 May 2024 2,416 3 View

Analyzing catch from gillnets with multiple mesh sizes?

Hello: I'm working on a database that includes catches from fishers who are stringing nets together with different mesh sizes. To reduce inconvenience for the fishers, the fish data were pooled....

22 May 2024 2,915 0 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM images?

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?

25 July 2024 5,482 2 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View

VECM Model: Positive/insignificant CointEq1 ?

Hello everyone, I am conducting

22 July 2024 8,085 11 View