James Smorenburg

3 Questions 1 Answers 0 Followers

Questions related from James Smorenburg

No bands at lower molecular weights for eWAT ponceau on nitrocellulose membrane?

Hi everyone I have been running some western blot samples on my eWAT. I run, transfer, block, primary, secondary, ECL and then image and ponceau after this. I was just wondering, when I ponceau...

13 March 2024 9,835 0 View

Gene expression: Cells Vs Tissue?

Hi Everyone I have been running QPCR for my tissue samples. On each plate I run a control which has NONO with cDNA made from cells. When I ran NONO for all my samples (+ control), the Cq values...

26 July 2023 2,941 0 View

3-Way ANOVA Graphpad?

Hi Everyone I am Graduate student who isnt the greatest at statistics and I have a question regarding a 3 way ANOVA. I am trying to run a 3-way ANOVA for a data set in graphpad that is the...

19 May 2023 592 2 View