Bacillus subtilis is a Gram-positive organism and has a simpler cell wall structure than E. coli, which is a Gram-negative organism. This makes it an ideal host for the expression of recombinant proteins and other foreign gene products. The simple cell wall structure of B. subtilis also means that it is more tolerant to the presence of foreign DNA, making it an attractive host for the expression of vectors with large inserts. Additionally, B. subtilis possesses a number of features that make it attractive for genetic engineering, including efficient cloning strategies and a well-developed toolbox of molecular biology techniques.
Udeshika Daya E.coli and Bacillus sp. were the first bacteria used for molecular and genetic manipulations. E.coli - 1946 (Gene recombination in E.coli. Nature, 158, 558) Bacillus - for lysogeny research (1950). Thus, the experience accumulated for both bacteria was similar and it is larger comparing to any other bacterium.
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