We are looking for antibodies that efficiently label neurons for FACS analysis (possibly in the nucleus). We tried an antibody against NeuN from Chemicon but it did not work for us.
Milli-Mark™ Anti-NeuN-PE, clone A60
http://www.millipore.com/catalogue/item/fcmab317pe
I think it works well.
Milli-Mark™ Anti-NeuN-PE, clone A60
http://www.millipore.com/catalogue/item/fcmab317pe
I think it works well.
The Monoclonal Antibody against Neuronal ClassIII ß-Tubulin (MMS-435P; Covance) works well.
Any specific neuronal type you are looking for ? Neuronal progenitors? Terminally differentiated ? Basic neural stem cells?
No specific neuronal type. We need to normalize quantifications done with a specific marker. Neuronal progenitors and/or terminally differentiated neurons would be fine.
You can try both early and late markers for normalization . Nestin being early marker for progenitors and Beta 3 tubulin for late (Terminal neurons). both markers are cytoplasmic not nucleus. chemicon anti bodies works fine for FACS. specificity to the source of your interest as to be verified before use.
did the procedure of staining NeuN involve permeablization? since its a nuclear localization 0.1% triton X for 30mins should do the trick.
mainly - Specificity of the antibody, Permeablization of the cells and cell type and the marker as to be verified
I have seen a review on Masanori's antibody that rate it as a very good one for IHC.
http://www.antibody-adviser.org/antibodies/view/17
I think it would be useful for anyone to begin to share his experience on antibodies in a centralised way.
Hi,
Does Milli-Mark™ Anti-NeuN-PE, clone A60 http://www.millipore.com/catalogue/item/fcmab317pe work on live neurons? or fixed ones?
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