I am trying to differentiate hiPSC-derived neurons. I noticed that at the very early stage, normally the first 3 days of differentiation, cells can be hardly observed. Day 5 onwards more neuron-like cells are developing, but they always clump together. Besides that, other than the neuron-like cells, there are another different morphology of cells appearing. Are potential neurons supposed to clump together while differentiating? Are the big cells without a very defined and obvious axonal processes some other cell types? I am using 96-well plate to differentiate neurons, could it be the 96-well plate has a round bottom which cells tend to accumulate in the centre?
They are tend to clump together but mostly further in their differentiation process. In your case it could be due to coating, you havent mentioned what kind of coating you used but increasing the concentration of biological matrix may help you.
They are tend to clump together but mostly further in their differentiation process. In your case it could be due to coating, you havent mentioned what kind of coating you used but increasing the concentration of biological matrix may help you.
They are tend to clump together but mostly further in their differentiation process. In your case it could be due to coating, you havent mentioned what kind of coating you used but increasing the concentration of biological matrix may help you.
They are tend to clump together but mostly further in their differentiation process. In your case it could be due to coating, you havent mentioned what kind of coating you used but increasing the concentration of biological matrix may help you.
They are tend to clump together but mostly further in their differentiation process. In your case it could be due to coating, you havent mentioned what kind of coating you used but increasing the concentration of biological matrix may help you.
They are tend to clump together but mostly further in their differentiation process. In your case it could be due to coating, you havent mentioned what kind of coating you used but increasing the concentration of biological matrix may help you.
They are tend to clump together but mostly further in their differentiation process. In your case it could be due to coating, you havent mentioned what kind of coating you used but increasing the concentration of biological matrix may help you.
They are tend to clump together but mostly further in their differentiation process. In your case it could be due to coating, you havent mentioned what kind of coating you used but increasing the concentration of biological matrix may help you.
It is pretty normal to get clusters of progenitors that give rise to neurons in a cluster like this. The small colonies and really tentative projection formation could be due to poor coating of the matrix you are using for the plates.
Try extending the coating period overnight before you use the plates. Also check the viability of your coating matrix. If it is temp sensitive make sure it has not gelled up before usage.
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