Teo Ting Ting

9 Questions 11 Answers 0 Followers

Questions related from Teo Ting Ting

How to prepare 10mM acetic acid and 20% ethanol?

I am trying to dilute the TGF-B3 growth factor with the recommended diluent: sterile-filtered liquid containing 10mM acetic acid and 20% ethanol. My questions: 1. How to prepare 10mM acetic...

17 May 2021 9,350 2 View

Anyone with experience in culturing Normal Human Astrocyte?? Are these cells healthy?

Dear All, I am new to this Normal Human Astrocyte (LONZA). They are primary culture. The first vial of cryopreserved cells that I revived seems ok, however, another does not. I noticed a big clump...

23 January 2021 4,778 2 View

Can qPCR used to quantify cells over other methods like ICC & flow cytometry?

I am currently working to generate a certain type of neurons. However not all the cells in the culture will be expressing the specific markers. I would like to check how many cells are expressing...

04 October 2020 5,078 2 View

Abnormal morphology of SNL76/7 fibroblasts of my culture, what has been wrong to them?

As I know SNL76/7 is a passage-independent feeder, hence I assume they will not change morphologically and karyotypically, after indefinite sub-passaging. However, recently I noted the changes in...

10 August 2020 9,799 6 View

Neuron Differentiation: Cells always clump together, is that a normal phenomenon?

I am trying to differentiate hiPSC-derived neurons. I noticed that at the very early stage, normally the first 3 days of differentiation, cells can be hardly observed. Day 5 onwards more...

29 July 2020 4,048 9 View

IPSCs which grow on feeder are not compacted colonies. Why?

I am totally new to feeder-dependent iPSC culturing. Previously I was doing on feeder-free iPSC culturing. Hence I encountered that the iPSCs which grow on the feeder are sparse and not growing as...

29 July 2020 8,772 4 View

Is Heat Inactivation of FBS always required before adding to medium?

So I have done once Heat Inactivation of FBS which is from an entirely new bottle, and then I prepared few aliquots with 5mL each. These few aliquots of FBS are most probably to be used within 2-3...

02 July 2020 4,121 3 View

How to calculate the population of each cell type in a culture?

I am trying to do immunocytochemistry to characterize the cells in a culture. As I am actually differentiating a type of neuron from neural stem cells, and I have done once the differentiation and...

05 June 2020 6,632 4 View

Is sterile-filtered solution safe for cell culture work?

I need to prepare or dilute TGF-B3 in the mentioned solutions: sterile-filtered 10mM acetic acid with 20% ethanol, or 4mM HCl. I have just searched through videos from YouTube, sterile filtration...

01 January 1970 8,345 3 View