Teo Ting Ting

6 Questions 9 Answers 0 Followers

Questions related from Teo Ting Ting

How to prepare 10mM acetic acid and 20% ethanol?

I am trying to dilute the TGF-B3 growth factor with the recommended diluent: sterile-filtered liquid containing 10mM acetic acid and 20% ethanol. My questions: 1. How to prepare 10mM acetic...

17 May 2021 9,259 2 View

Can qPCR used to quantify cells over other methods like ICC & flow cytometry?

I am currently working to generate a certain type of neurons. However not all the cells in the culture will be expressing the specific markers. I would like to check how many cells are expressing...

04 October 2020 4,920 2 View

Abnormal morphology of SNL76/7 fibroblasts of my culture, what has been wrong to them?

As I know SNL76/7 is a passage-independent feeder, hence I assume they will not change morphologically and karyotypically, after indefinite sub-passaging. However, recently I noted the changes in...

10 August 2020 9,680 6 View

Neuron Differentiation: Cells always clump together, is that a normal phenomenon?

I am trying to differentiate hiPSC-derived neurons. I noticed that at the very early stage, normally the first 3 days of differentiation, cells can be hardly observed. Day 5 onwards more...

29 July 2020 3,966 9 View

Is Heat Inactivation of FBS always required before adding to medium?

So I have done once Heat Inactivation of FBS which is from an entirely new bottle, and then I prepared few aliquots with 5mL each. These few aliquots of FBS are most probably to be used within 2-3...

02 July 2020 3,983 3 View

Is sterile-filtered solution safe for cell culture work?

I need to prepare or dilute TGF-B3 in the mentioned solutions: sterile-filtered 10mM acetic acid with 20% ethanol, or 4mM HCl. I have just searched through videos from YouTube, sterile filtration...

01 January 1970 8,273 3 View