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Questions related from Teo Ting Ting
I am trying to dilute the TGF-B3 growth factor with the recommended diluent: sterile-filtered liquid containing 10mM acetic acid and 20% ethanol. My questions: 1. How to prepare 10mM acetic...
17 May 2021 9,277 2 View
Dear All, I am new to this Normal Human Astrocyte (LONZA). They are primary culture. The first vial of cryopreserved cells that I revived seems ok, however, another does not. I noticed a big clump...
23 January 2021 4,722 2 View
I am currently working to generate a certain type of neurons. However not all the cells in the culture will be expressing the specific markers. I would like to check how many cells are expressing...
04 October 2020 4,948 2 View
As I know SNL76/7 is a passage-independent feeder, hence I assume they will not change morphologically and karyotypically, after indefinite sub-passaging. However, recently I noted the changes in...
10 August 2020 9,720 6 View
I am trying to differentiate hiPSC-derived neurons. I noticed that at the very early stage, normally the first 3 days of differentiation, cells can be hardly observed. Day 5 onwards more...
29 July 2020 3,978 9 View
So I have done once Heat Inactivation of FBS which is from an entirely new bottle, and then I prepared few aliquots with 5mL each. These few aliquots of FBS are most probably to be used within 2-3...
02 July 2020 4,010 3 View
I am trying to do immunocytochemistry to characterize the cells in a culture. As I am actually differentiating a type of neuron from neural stem cells, and I have done once the differentiation and...
05 June 2020 6,560 4 View
I need to prepare or dilute TGF-B3 in the mentioned solutions: sterile-filtered 10mM acetic acid with 20% ethanol, or 4mM HCl. I have just searched through videos from YouTube, sterile filtration...
01 January 1970 8,284 3 View
