Dear all,
I need a protocol for purification of thioredoxine fused protein with Ni-NTA column from bacterial system.
if any one have the protocol please share.
Thank you
Ragards
B.Venkatesh
I have a basic protocol which I used for TRX-fused mutatant protein (although it did not work for me but I got it from a Trx lab)
Here it goes.
1. I tried expressing Trx mutants in Origami DE3/ Shuffle T7 cells
2. Grow cells untill OD 0.6 in LB (with antibiotic) and 2% Glucose
3. Incubate on ice for 30 mins (induces cold shock proteins)
4. Induce with 100uM IPTG
5. shake O/N at 16-18 degrees...
hope this works for you
best
Sneha
thank you for your reply sneha madam
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