Hi everyone
I am currently investigating the effect of iron deficiency on neuronal cells (SHYSY5Y to be exact). For this, I need to create an environment of iron-depletion in-vitro. While past publications have used iron chelators in media, this involves considerable quality control. This is why I am choosing to mimic an iron-deficient environment through serum starvation.
I am currently struggling to find publications outlining a validated method of serum starvation to achieve this, which would be of great help, as a trial- and error method in the lab is time consuming. Another concern is that other essential nutrients would also be depleted with serum starvation, that may affect any findings and therefore impact the validity of results. I am also interested in any iron-depleted media out there that I could potentially use?
Would greatly appreciate any advice, links to publications or methods I could follow.
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