Need Protocol for LPS induced inflammation in HaCaT cells?

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Which solution reveals the microstructure and features of nickel-based alloys?

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Which is the most convenient manufacturing software for Additive Manufacturing?

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Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

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How does one access the Cranet survey data on HRM practices?

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Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

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Can the problems of overfitting be solved without cross validation?

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Why do I see variability in mRNA expression in qPCR of the same gene (ubiquitin)?

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Immunostaining of common bacteria in FFPE samples?

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Experience measuring human LPS in plasma?

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15 November 2020 6,599 1 View

What's the best way to detect pro-inflammatory cytokines in RAW264.7 supernatant?

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Do you have any suggestion for a easy and cheap experiment to quantify Cytokines?

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Determination of Lipid A in Salmonella?

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Does LPS elicit an inflammatory response in the neutrophil-like cell line HL-60?

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What is the volume of LPS should be added in each well to induce changes in RAW 264.7 cells using 24 well plates?

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Yanting Wu

Dear Dinesh,

Maybe you can have a look at this paper for reference,

Anti-Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study, 2015

Roberta Di Caprio, Serena Lembo, Luisa Di Costanzo, Anna Balato and Giuseppe Monfrecola

Best wishes

Dinesh Kumar

Thanks Dear Yanting for the article you recommended. It has clearly stated the procedure.

Regards