Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

How do I quantify lead in tissue?

Hello everyone! I'm looking for some novel ways to measure out the amount of lead shot spray found in wildlife tissue. I have radio-graphed some deer ribs and gut piles left by hunters. They are...

24 January 2021 2,150 7 View

Why am I getting RNAse P in my negative extraction?

When performing Viral RNA extractions I perform a negative extraction using water. This is then run as a negative control for my RT-PCR reaction. Recently this control has been showing up RNAse P...

07 July 2016 2,309 3 View

Does anyone have experience developing ELISA kits?

We want to develop an ELISA that can detect antibodies to certain bacteria in serum samples. I have no idea how to start developing my own ELISA so if you have suggestions that would be wonderful.

22 February 2013 6,445 11 View

I'm having difficulties getting the Interplate variability on an ELISA to below 15%CV.

I am very precise with my pipetting so I'm wondering if it's my timing with each plate. I am working with a sensitive CAYMAN kit testing for corticosterone. Does anyone have some insight on these...

17 February 2013 4,944 13 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

What is the role of fresh cow dung in sprouting in stem cutting propagation?

In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.

24 December 2020 4,068 6 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View

How to cut dsDNA chain with Uracil-DNA Glycosylase at the point of dU in room temperature?

18 December 2020 6,908 3 View

Why pink coloured or light red onion have more storage life than white and dark red one and smaller size one to has more than bigger one?

It is generally seen light coloured varieties has more storage life.what's relation with colour and size with storage life.

13 December 2020 634 18 View

Can LAMP distinguish the species from different genus/family?

I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...

07 December 2020 5,630 3 View