Can you perform the Murexide test on samples fixed in Formalin?

23 February 2021 1 2K Report

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

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How do I quantify lead in tissue?

Hello everyone! I'm looking for some novel ways to measure out the amount of lead shot spray found in wildlife tissue. I have radio-graphed some deer ribs and gut piles left by hunters. They are...

24 January 2021 2,150 7 View

In this sterile needle biopsy wet-mount, does the motility pattern fit with Oomycete zoospores?

We are examining tissue from a cluster of human and canine patients with a similar pattern of systemic illness of unknown cause. All members of the cluster have evidence of motile zoospore-like...

23 October 2020 4,480 3 View

These images may show a novel/emerging human pathogen- Do you think it is consistent w/ oomycetes/myxomycetes/fungus? Anyone pursuing this already?

I am a member of a group of veterinarians who all became ill within about a years time of each other, and who all were employed in the same physical location. Some of the affected veterinarians...

02 June 2020 2,248 19 View

Which housing is better for testing typical mouse behaviors: housing one genotype together or mix in littermate controls?

There's a debate in my lab currently on the best housing practice for neurobehavior. Should a cage contain a mixture of mice (ie; WT, KO, KO-treated) or only one (ie; KO-treated). I am curious...

26 March 2020 3,061 4 View

Why is my clear microemulsion and clear gel base when combined become turbid/cloudy?

So, I'm formulating a microemulgel. the appearence of my microemulsion and gel base was clear and transparant. but when i combine the two phases, the microemulgel becomes cloudy. what could...

10 March 2020 6,433 4 View

Hi, what's really the droplet size range of microemulsion?

Recently, i made o/w microemulsion with stirring at 600 rpm. and it resulted to be clear, transparant, almost like water (the transparancy), a bit yellowish. but the droplet size is 300 nm whereas...

10 March 2020 7,022 3 View

Does anyone have even a guess at what type of organism (phylum/class?) the following electron micrographs might show?

We have obtained some electron micrographs showing what appears to be developmental stages of some type of small eukaryotic organism colonizing/parasitizing the tissues of mammalian hosts....

03 November 2019 2,041 2 View

I would be grateful for any opinions about the identity or characterization of this object. (Artifact vs. organism etc..)?

Full history is in the project description, but briefly- a team of other veterinarians/colleagues and myself have stumbled across a case of multiple animals that have similar symptoms and are...

18 September 2019 1,000 4 View

Field CA3/CA1 electrophysiology differences between rats and mice?

I've been studying field hippocampal electrophysiology in mice for many years and we've recently started working in rats. I am beyond frustrated because things do not work at smoothly in rats and...

09 February 2019 6,713 8 View

Best ACSF recipe for LTD (DHPG AND LFS)?

Hi everyone! We typically record from rat acute hippocampual slices for LTP but I also need to expand my study into long term depression. After I ran a couple experiments, I completely forgot...

09 May 2018 858 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View