To start of I am trying to isolate DNA from blood and ticks for the identification of the tick species and a parasite that is transmitted by the ticks, by running it through agarose gel, and a limited budget.
To be clear about this question, I get no DNA from Rhipicephalus decloratus ticks when using the Kapa Express Extract kit that can extract DNA from insects. I have followed the instructions to the letter. The only unclear thing in the instructions is that i should spin at high speed for 1 min.
Can anyone please help me by telling me perhaps at what speed this is(rpm or xg).
Alternatively if someone has experience with this kit and ticks please provide me with a few pointers or alterations to the protocol to get the maximum amount of DNA (dilution is a possibility afterwords)
And please remember that this is before the PCR is performed (which does not work due to no DNA).
Hi, I suggest crushing the insect in a separate tube with liquid nitrogen before you go through DNA extraction procedure and Increase lysis time to 30 min to improve release of DNA.....good luck
Hello, My suggestion to you is to use liquid nitrogen while grinding the samples if you are not. this might help you with that issue..
Hi, I suggest the DNA extraction at high speed and in a short time and then rinse several times with sterile water after the kit is used. good luck
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