Rhizosphere sampling techniques in sandy soils?

I need to sample soil around 2 m high shrubs - so no 'pullup the plant and shake of the soil' will work. These plants have taproot systems of 2m deep. My idea is to compare the microbes in the...

29 April 2019 8,556 3 View

Can I do a group search for metabolites associated with certain proteins?

I have a list of proteins and would like to know what metabolites are associated with each protein. I can manually run them through the human metabolome database, but it is time consuming. So I...

07 February 2019 5,013 2 View

Information on sediment loads in KZN rivers?

Although information on total annual sediment loads is available I have been unable to find information on how seddment loads are distributed throughout a typical hydrological year.

16 September 2018 1,013 3 View

How to settle clay particles in soil solution for Biolog Ecoplate?

I have claye soils that I want to use Biolog Ecoplates on. Can I use CaCl2 (1ml of 1M in 100 ml of 10% soil solution) to settle the soil? Or maybe centrifuge (5000 rpm for 1 min)? I do not want...

31 October 2017 9,482 1 View

Any reasons for low Vitamin A (palmitate) recoveries from Infant Milk Formula?

23 August 2016 2,849 5 View

Does anyone in the US have the HCC2157.3 breast carcinoma cell line?

Seeking advice.

23 May 2014 4,029 1 View

Need help in extracting DNA from ticks using Kapa Express extract kit?

To start of I am trying to isolate DNA from blood and ticks for the identification of the tick species and a parasite that is transmitted by the ticks, by running it through agarose gel, and a...

01 January 1970 1,451 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View

Is there a surface inactive water-soluble dye?

Hi guys, I'm looking for a water-soluble dye which is not surface inactive in order to observe (water in oil) emulsions; I don't prefer to use oil-soluble dyes. I've used rhodamin123,...

21 January 2021 8,496 2 View

Can anyone suggest an RNA extraction protocol from grape berries?

I am trying to extract RNA, from grape berries of a red variety, for qPCR (to study gene expression). I have tried nucleozol and a CTAB protocol (with b-mercaptoethanol+boric acid + cleaning with...

18 January 2021 8,857 2 View

No title

16 January 2021 8,334 5 View