Hi,
I am working on compound identification using HPLC. Each compound has its unique Uv-Vis spectrum. Here are some compounds that are creating trouble for me and I can’t recognize their spectrum. I am attaching these spectra. Please let me know if someone is familiar with those spectra. I’ll be highly obliged.
Thank you very much.
UV-VIS spectrum is not very specific information for chemical, in HPLC, it can use as assistant information to confirm the chemical with retention time. In general, standard or reference material should be analysis in your HPLC system to get the right RT and UV-VIS spectrum in your system.
You can't identify compounds with UV-Vis Spectra unless you have already analyse the standards and have the same combination (Retention time + spectra).
Sometimes you can use UV-Vis spectras to have an idea of the spectra of related compouds (for example in a same family).
In fact characterization of unknowns compounds with only UV-Vis detector is impossible.
Remember to combine the info from UV det. - tje presence of chromofore groups with retention. Make additional runs - change the pH to check if your compound is ionisable or not. This, including the info anout the sample origin can give you some hint. At tje end you can make semi-preparativ separation - collect the fraction and make different analysis like FTIR or NMR.
regards
G
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