Muhammad Safiullah Virk

5 Questions 8 Answers 0 Followers

Questions related from Muhammad Safiullah Virk

Need help for compound identification via Uv-Vis spectra?

Hi, I am working on compound identification using HPLC. Each compound has its unique Uv-Vis spectrum. Here are some compounds that are creating trouble for me and I can’t recognize their spectrum....

06 June 2018 6,284 3 View

Can pBR322 ORI be used in yeast plasmids?

Hi, as pBR322 is one of the oldest plasmids used. Can pBR322 be used in the construction of a yeast plasmid or can a vector having pBR322 ORI and BOM be assembled in yeast? Thank you for the answers.

08 August 2017 2,670 2 View

Can someone help in EHA105 Colony PCR?

Hello Everybody, Hope you doing good.. I am facing the problem in colony PCRof Agrobacterium EHA105, I did it abou20 times but failed every time. Can any body tell me the best way to deal with...

12 December 2015 8,646 4 View

What is the fate of suicide plasmid in fungi gene knockout or gene silencing? Does it keep growing with generations or is it removed?

Hello Everyone, I need to know about the difference of fate of suicide plasmid in fungi and in bacteria.. As I know, in bacteria, suicide plasmid deletes a gene and is removed in next generations,...

12 December 2015 5,831 3 View

What is fate of introns during splicing?

The main question i want to know is, is every intron have its own machinary yo be removed or machinary for intron control is some where upstream of the gene? Secondly, bioinformatic analyses for...

12 December 2015 1,437 1 View