Need a topic to study on RNA from human blood where i can utilise QIAamp RNA Blood MINI KIT?
This may sound a bit strange but I am looking for a good study topic where i can utilize Qiagen Qiaamp RNA Blood Mini Kit for some good use.
Hello Everyone
The thing is i am working in a district government hospital as a Microbiologist. Here along with routine sample analysis we are also developing a molecular microbiology lab where we are developing protocol for identification of human pathogens and detecting drug resistance. We have a QIAGEN QIAcube, Rotor Gene Q, QIAxpert, Serum HPLC and PCR at our disposal.
Now during our initial setup we had asked QIAGEN to provide all the necessary kit which would be required by our lab. That's where they provided us with 4 Boxes, 50 reaction each of Qiagen Qiaamp RNA Blood Mini Kit. We are not sure where to put good use of it. We are still not studying human blood RNA. We do study viral RNA but for that we use QIAamp Viral RNA mini kit.
Now since we have this RNA Blood kit, we are planning to use it for some useful purpose which would help further development of our laboratory. We have an attached pathology lab where we can get blood sample for any disorder.
So i need help from you on suggesting me based on your experience
a) a topic which i can look upon or
b) a target RNA in human blood for disorder/Cancer or
c) Any Viral RNA which can be recovered by Qiagen Qiaamp RNA Blood Mini Kit from blood or
d) Any other suggestion
by which i can utilize RNA Blood Mini Kit
Limitation: I am looking for a single or may be just 2 RNA target to study. We have Reverse transcriptase kit and Q-PCR at our disposal. We can also purchase primer for a any target CDNA. Designing a single probe for Q-PCR is possible but i don't have budget for multiprobe study. If the target gene could be studied by SYBR green kit than that would be great.
Thank you
Limitation: I am looking for a single or may be just 2 RNA target to study. We have Reverse transcriptase kit and Q-PCR at our disposal. We can also purchase primer for a any target CDNA. Design probe for Q-PCR is possible but i don't have budget for multiprobe study. If the target gene could be studied by SYBR green kit than that would be great.
Hi Mustafa,
First, you need to select the disease based on the sample availability. And then the second step will be to identify the gene targets for RNA experiments, this can be done in silico using the publicly available gene expression datasets (however this requires bioinformatic skills). Other way to narrow down to the targets may be to find out some good publication (literature survey) which have identified novel target genes for the particular disease but the experimental validation is yet to be done on human samples and use these target genes for your RNA experiments after acknowledging that particular article.
Hope this helps.
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