I am looking for a protocol to stain yeast cells with PI and DAPI in order to identify dead cells with confocal microscopy.
Will this help? Article Insights into the intracellular mechanisms of citronellal in...
When I am importing the orbit file created using STK into OPNET, I am getting the following error: Unable to import STK orbit file (C:\Users....). How can I fix this?
11 March 2023 5,020 2 View
I have read that early administration of ART in cases effected with cryptococcal meningitis lead to a higher level of mortality but unfortunately could not get a clear understanding about the...
05 January 2019 8,688 0 View
31 December 2018 7,124 0 View
I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal or a potent antifungal drug candidate? Is there any...
13 October 2018 7,793 3 View
09 October 2018 10,051 4 View
10 August 2018 2,451 1 View
I am testing plant product against a yeast and want to determine the minimum inhibitory concentration of the same against the fungi. It has not yet been used against the said yeast. What...
06 July 2018 1,181 1 View
I want to visualize the effect of a drug on the plasma membrane of the yeast cell. What are the fluorescent markers that I can employ for this?
01 February 2018 5,042 2 View
I am working on fungal infections in rodents. I am interested in knowing that what might be the probable reason of lowering of the MDA levels in case of infected groups and increase of the same...
13 October 2017 2,250 0 View
09 October 2017 2,476 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View