How does Anti-retroviral therapy effect cryptococcal meningitis?

I have read that early administration of ART in cases effected with cryptococcal meningitis lead to a higher level of mortality but unfortunately could not get a clear understanding about the...

31 December 2018 7,040 0 View

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal?

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal or a potent antifungal drug candidate? Is there any...

13 October 2018 7,718 3 View

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal?

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal or a potent antifungal drug candidate? Is there any...

09 October 2018 9,955 4 View

Need a protocol for PI and Dapi staining to identify dead cells for confocal microscopy?

I am looking for a protocol to stain yeast cells with PI and DAPI in order to identify dead cells with confocal microscopy.

10 August 2018 2,365 1 View

How to decide an antimicrobial's concentrations to be used to check susceptibilities if it has not yet been used against the organism of interest?

I am testing plant product against a yeast and want to determine the minimum inhibitory concentration of the same against the fungi. It has not yet been used against the said yeast. What...

06 July 2018 1,111 1 View

Can any one tell me about efficient fluorescent plasma membrane markers for yeast cell?

I want to visualize the effect of a drug on the plasma membrane of the yeast cell. What are the fluorescent markers that I can employ for this?

01 February 2018 4,937 2 View

What might be the probable reason of Malondialdehyde level going down in case of fungal infection and rising after treatment comparing with control?

I am working on fungal infections in rodents. I am interested in knowing that what might be the probable reason of lowering of the MDA levels in case of infected groups and increase of the same...

09 October 2017 2,389 0 View

I need to install GROMACS on windows 10, 64 bit system. Can anyone help me with a step by step tutorial?

08 September 2017 1,011 16 View

What is the most feasible method for protein sequencing and where in India can it be done?

Protein sequencing

09 October 2015 7,838 0 View

Why are Minimum inhibitory concentration indicated as MIC 50 or MIC 80 or some times MIC 90?

Which one should be determined and what is the basis of selection? Minimum inhibitory concentration against fungi

05 June 2015 9,011 23 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View