How does Anti-retroviral therapy effect cryptococcal meningitis?

I have read that early administration of ART in cases effected with cryptococcal meningitis lead to a higher level of mortality but unfortunately could not get a clear understanding about the...

31 December 2018 7,040 0 View

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal?

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal or a potent antifungal drug candidate? Is there any...

13 October 2018 7,718 3 View

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal?

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal or a potent antifungal drug candidate? Is there any...

09 October 2018 9,955 4 View

Need a protocol for PI and Dapi staining to identify dead cells for confocal microscopy?

I am looking for a protocol to stain yeast cells with PI and DAPI in order to identify dead cells with confocal microscopy.

10 August 2018 2,365 1 View

Need a protocol for PI and Dapi staining to identify dead cells for confocal microscopy?

I am looking for a protocol to stain yeast cells with PI and DAPI in order to identify dead cells with confocal microscopy.

07 August 2018 4,631 1 View

How to decide an antimicrobial's concentrations to be used to check susceptibilities if it has not yet been used against the organism of interest?

I am testing plant product against a yeast and want to determine the minimum inhibitory concentration of the same against the fungi. It has not yet been used against the said yeast. What...

06 July 2018 1,111 1 View

What might be the probable reason of Malondialdehyde level going down in case of fungal infection and rising after treatment comparing with control?

I am working on fungal infections in rodents. I am interested in knowing that what might be the probable reason of lowering of the MDA levels in case of infected groups and increase of the same...

09 October 2017 2,389 0 View

I need to install GROMACS on windows 10, 64 bit system. Can anyone help me with a step by step tutorial?

08 September 2017 1,011 16 View

What is the most feasible method for protein sequencing and where in India can it be done?

Protein sequencing

09 October 2015 7,838 0 View

Why are Minimum inhibitory concentration indicated as MIC 50 or MIC 80 or some times MIC 90?

Which one should be determined and what is the basis of selection? Minimum inhibitory concentration against fungi

05 June 2015 9,011 23 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View