My cell line is recommended to be grown in 4mM glutamine,4500mg/l glucose and 1500mg/l sodium bicarbonate but I am growing in 2mM glutamine,2000mg/L glucose and 2000mg/l bicarbonate. will it affect the growth of my cell??
Arun,
Most of the cancer cell lines grow in different medium compositions. Medium preference is determined by the source of the cell line. In our lab we prefer ATCC standard medium composition for them. But some times it becomes obvious to alter medium( either components or total medium like DMEM to RPMI/MEM) where you have to observe the morphological changes for some generations, then only go for experiments. Apart from that Estrogen/Androgen/Insullin/EGF/FGF/Cholera toxin like growth regulators are essential for particular cell lines which you should not forget to include in new medium composition.
For instance, MCF-7 is recommended to grow in EMEM and 0.01mg/ml of Insulin by ATCC. But, I observed in many papers that MCF-7 growing in MEM/RPMI/DMEM(glucose levels 1g, 2g, 4.5g per liter, phenol red quantities varies respectively etc.). To avoid confusion its better to stick to cell line source composition.
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