How can I dry glycerol on nitrocellulose membrane without damaging antibody?

03 April 2017 3,379 1 View

Why are the gold-nanoparticles conjugated with a primary antibody (+ mixed with antigen) avoiding the primary antibody on nitrocellulose membrane?

I conjugated gold nanoparticles with a primary antibody and mixed it with the corresponding antigen. I ran the solution over a nitrocellulose membrane with two dots on it (one with the same...

02 March 2017 1,714 1 View

Should I store my antibody in 100 mM Borate Buffer with 1 % (w/v) BSA, PBS or something else?

03 April 2016 974 0 View

How do I create Washing Buffer (5 mM BB, 1% (w/v) BSA and Storage Buffer (Sb, 100 mM BB, 1% (w/v))?

I already have the 5 mM Borate Buffer and the 100 mM Borate Buffer. I also have 10% BSA. How do I create 5 mL of the WB and 5 mL of the SB? I need these two buffers to couple carbon nanoparticles...

03 April 2016 9,238 3 View

At what setting should I sonicate my carbon nanoparticles?

I will be using the M220 or E210 Covaris sonicator. Any insight would be greatly appreciated! I will be sonicating 1 mg of carbon nanoparticles in 0.1 mL of water and then later with added borate...

03 April 2016 5,339 3 View

What concentration of the antibody/antigen should I put on my control/test line?

I am currently developing a Lateral Flow Assay, and I have an antigen with a concentration of 1 mg/mL and a molecular weight of 20.7 kDA. I also have an antibody with a concentration of 2 mg/mL...

03 April 2016 8,477 11 View

How should I dilute my antigen and antibodies for my test and control line on LFA?

I need to pipet a low molecular weight antigen (around 20 kDA) on my test line and a secondary antibody of anti-rabbit goat antibody- IgG (around 120-160 kDA) on my control line. The...

03 April 2016 1,961 3 View

Is it necessary to add BSA to wash buffer?

03 April 2016 8,997 6 View

Is it possible to conjugate antibodies to Acid Black 48 Dye?

I know that because Acid Black 48 is cationic and the antibody is anionic, those two could non-covalently bind. Would this hypothetically work by allowing them to bind through mixing at 4 degrees...

03 April 2016 8,433 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View