My antibody contains 0.01% sodium azide. Would it be acceptable if I biotinylated the antibody with this concentration of sodium azide?

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Syed A Ali

Why to take risk; simply dialyze your antibody to get rid of sodium azide and proceed for biotinylation.

Lola Y.

I only have 25-40 microliters of my antibody, so I believe that dialysis would not work.

Well, what is the concentration of antibody? Even after biotinylation, you got to dialyze to remove free biotin.

The concentration is 1 mg/mL