Lola Y.

10 Questions 15 Answers 0 Followers

Questions related from Lola Y.

How can I dry glycerol on nitrocellulose membrane without damaging antibody?

Hi, I am pipetting an antibody with 50% w/v glycerol. However, I need the antibody solution to dry on the nitrocellulose membrane. How can I do this without damaging the antibody? Thank you.

04 April 2017 3,467 1 View

Why are the gold-nanoparticles conjugated with a primary antibody (+ mixed with antigen) avoiding the primary antibody on nitrocellulose membrane?

I conjugated gold nanoparticles with a primary antibody and mixed it with the corresponding antigen. I ran the solution over a nitrocellulose membrane with two dots on it (one with the same...

03 March 2017 1,767 1 View

Is it necessary to add BSA to wash buffer?

Hi, I know that BSA is commonly used to prevent non-specific binding. However, I was wondering if it is essential to be included in wash buffer that will be used to attach carbon nanoparticles to...

04 April 2016 9,052 6 View

At what setting should I sonicate my carbon nanoparticles?

I will be using the M220 or E210 Covaris sonicator. Any insight would be greatly appreciated! I will be sonicating 1 mg of carbon nanoparticles in 0.1 mL of water and then later with added borate...

04 April 2016 5,389 3 View

Should I store my antibody in 100 mM Borate Buffer with 1 % (w/v) BSA, PBS or something else?

Before storage, I am coupling my antibody with carbon nanoparticles using 5 mM Borate Buffer as my washing buffer. Here is some info on my antibody: Concentration- 1.0 mg/mL by UV absorbance at...

04 April 2016 1,041 0 View

How do I create Washing Buffer (5 mM BB, 1% (w/v) BSA and Storage Buffer (Sb, 100 mM BB, 1% (w/v))?

I already have the 5 mM Borate Buffer and the 100 mM Borate Buffer. I also have 10% BSA. How do I create 5 mL of the WB and 5 mL of the SB? I need these two buffers to couple carbon nanoparticles...

04 April 2016 9,307 3 View

Is it possible to conjugate antibodies to Acid Black 48 Dye?

I know that because Acid Black 48 is cationic and the antibody is anionic, those two could non-covalently bind. Would this hypothetically work by allowing them to bind through mixing at 4 degrees...

04 April 2016 8,473 2 View

What concentration of the antibody/antigen should I put on my control/test line?

I am currently developing a Lateral Flow Assay, and I have an antigen with a concentration of 1 mg/mL and a molecular weight of 20.7 kDA. I also have an antibody with a concentration of 2 mg/mL...

04 April 2016 8,527 11 View

How should I dilute my antigen and antibodies for my test and control line on LFA?

I need to pipet a low molecular weight antigen (around 20 kDA) on my test line and a secondary antibody of anti-rabbit goat antibody- IgG (around 120-160 kDA) on my control line. The...

04 April 2016 2,004 3 View

My antibody contains 0.01% sodium azide. Would it be acceptable if I biotinylated the antibody with this concentration of sodium azide?

Has anyone tried to biotinylate an antibody with a similar concentration of sodium azide? I only have 25-40 microliters of my antibody, so I believe that dialysis would not work in this case.

04 April 2016 8,053 4 View