Murine NK cell line?

More Ioannis Kokkinopoulos's questions See All

Is there a competent body to certify and judge whether a new anti-seismic technology makes a positive contribution or whether it is dangerous?

21 June 2024 9,919 3 View

How do we reduce the tensile force on the side of the wall without prestressing?

There is the torque on the wall, which tends to rotate it. As it rotates, the diagonal forces down, the ground resists and compression is created. The other side of the wall, raises forces which...

23 March 2024 8,422 3 View

What are our homes at risk from?

1. Fire 2. Earthquake 3. Flood But the big bill comes from 4. not maintaining them. How can we maintain the structures if we can't? Because the buildings will start falling down on their...

21 March 2024 3,362 1 View

Does anyone have the following article "Canine haematological changes during gestation and lactation. " ?

Authors: R. L. Allard, A. D. Carlos, E. C. Faltin Journal Article : Companion Animal Practice , Vol.19, No.3, pp.3-6

11 February 2024 8,690 1 View

What is better, the earthquake forecast, or building strong earthquake structures?

To deal with a strong earthquake what is best. 1. To anticipate the earthquake and flee our homes to avoid being killed, or 2. To build strong structures unscathed in the earthquake ?

03 February 2024 6,576 15 View

Is there a Shadowban in scientific journals for the papers we publish?

Is there a Shadowban in scientific journals for the papers we publish? Shadowban is the practice of blocking or partially blocking a user or their content from certain areas of an online community...

15 January 2024 6,604 7 View

Are extreme natural phenomena more frequent lately?

Dear colleagues Good morning. I have noticed that in the few recent years extreme natural phenomena are more frequent than before (see file attached). Is this assumption correct? Please comment...

14 January 2024 495 9 View

What is the world record for traction on ground and rock anchors?

There are many anchoring mechanisms for soil and rock. Some resist traction, others resist compressive loads What is the world record and what is the strongest traction anchorage known in soil...

17 December 2023 8,409 4 View

How can I separate Diglycerol and Diglycerol Dicarbonate from a mixture using HPLC?

In literature, they separate the simpler form of the mentioned compounds, Glycerol and Glycerol Carbonate by GC. I tried GC but I could not separate them so I decided to try HPLC. However, I have...

06 December 2023 4,992 3 View

Is the "Lamprophyre Clan" or the "Lamprophyre Facies" correct?

Dear colleagues Good day to you all. One of the most famous debates in Igneous Petrology is the relation between the diamondiferous rocks (i.e. lamprophyres, lamproites, orangeites and...

05 November 2023 813 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Yasin Ilgaz

You may check these

https://www.kerafast.com/p-2099-natural-killer-cell-line-kil.aspx

https://www.lgcstandards-atcc.org/products/all/CRL-2407.aspx?geo_country=tr

Ioannis Kokkinopoulos

many thanks for your prompt answer!

Philippe Joly

second possibility : normally you can buy at the Karolinska Institute in sweden