Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Permanganate oxidizable carbon pH adjustment ?

03 February 2021 1,706 2 View

How can I track the decrease in initiation events of combustion?

Hello, I am interested in tracking the decrease in initiation events of different wildland fuel combustion. Apart from the experimental procedures, is there any simulation software that can help...

12 October 2020 7,905 1 View

Interpretation of Posterior distribution produced by MCMCglmm?

Dear all, I have a comparative phylogenetic model on MCMCglmm, with a binary response variable, 4 binary explanatory variables as fixed effects and the phylogeny as a random effect. I would...

29 September 2020 2,165 2 View

How to analyze differences in short-, mid-, and long-term improvements between two groups?

Hello folks! We created two training courses: A (N=25) and B (N=25). Participants were assigned to one of the two groups respectively. They were tested before, shortly after, and 3 months after...

13 July 2020 2,903 3 View

Did anyone find the right input for mush parameter (Amush) in solidification and melting model of ANSYS FLUENT?

Hello! I am currently involved in a Direct Chill Casting Model of an Aluminum Alloy. The model was built both in ANSYS FLUENT and in COMSOL. I am facing convergence issues and i cannot find the...

23 April 2020 8,253 1 View

Is it possible to couple fluid flow, heat transfer, phase change and mechanical stresses on the solidifying shell using COMSOL Multiphysics?

I am trying to simulate DC Casting of Aluminum Alloys in Comsol. I found an example related to Continuous Casting that it couples fluid flow, heat transfer and phase change during solidification...

28 March 2020 3,010 4 View

Is there a protocol for a multicolour fluorochrome cell staining in microplates?

Our laboratory is not equipped with a flow cytometer. Is it possible to stain cell suspensions (i.e. from thymus) in 96 well plates with multiple fluorochrome conjugated antibodies and then read...

23 February 2020 1,566 3 View

BaysTraits analysis: Using random sample of trees or most probable trees?

Hello. I want to run an MCMC analysis on BayesTraits using a set of Bayesian trees I created using MrBayes. I want to run the analysis using both the consensus tree and a sample of 1,000 trees to...

28 July 2019 5,860 2 View

Can you specify the gene type in a partition file?

Hi! I am trying to build a phylogeny using a mixture of mitochondrial and nuclear protein-coding genes and rRNA genes. Is it possible to specify the specific type of gene in the partition file? I...

16 December 2018 6,033 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View