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Questions related from Ioannis Kokkinopoulos
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
04 March 2021 4,144 1 View
I am getting some weird results when transplanting human peripheral blood CD34+ cells into humanised mice. After several weeks, the human cells in the bone marrow are CD45+ indeed, but there is a...
19 February 2020 1,070 3 View
Hello all, I have performed a number of experiments in differentiating my mESCs, using negative controls (unstained) plus single-stain for each of the Abs used for proper compensation. Yet, when...
13 April 2019 7,852 11 View
I was wondering if anybody knows what is the half-life of 4(OH)TAM in vitro? I am adding 4(OH)TAM at 0.5ng/mL in my murine ESC cultures every 24 hours for 5-6 days and I was wondering if this is...
28 March 2019 7,905 2 View
I am currently looking into reviving T-cells or B-cells from frozen human tissue. Is it feasible? If yes, is there a specific way on how to freeze the tissue in the first place to get the best...
11 November 2016 1,372 6 View
I am currently looking for a murine NK cell line to use, with no avail. Is there anyone that may have one to provide or point to the direction of getting one? Thank you
03 November 2016 3,586 3 View