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Questions related from Ioannis Kokkinopoulos
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
04 March 2021 4,402 1 View
I am getting some weird results when transplanting human peripheral blood CD34+ cells into humanised mice. After several weeks, the human cells in the bone marrow are CD45+ indeed, but there is a...
19 February 2020 1,129 3 View
Hello all, I have performed a number of experiments in differentiating my mESCs, using negative controls (unstained) plus single-stain for each of the Abs used for proper compensation. Yet, when...
13 April 2019 7,919 11 View
I was wondering if anybody knows what is the half-life of 4(OH)TAM in vitro? I am adding 4(OH)TAM at 0.5ng/mL in my murine ESC cultures every 24 hours for 5-6 days and I was wondering if this is...
28 March 2019 7,978 2 View
The manufacturer indicates up to 10 days, but I think this is too short. How long does it last, at 4C, and able to keep cells undifferentiated from your mESC culture experience? Has anyone...
01 March 2019 5,551 0 View
A bit of help from the experts. We had a fresh human bone marrow sample (in EDTA), delivered to us, which we diluted 1:3 in (1x) PBS/2mM EDTA prior to loading it onto Histopaque 1077, centrifuging...
02 November 2017 7,626 0 View
I performed a preliminary mitochondrial assay using Wild-type and ApoE KO Sca1+ cells, fed with glucose or galactose. Under both conditions, the ApoE KO cells showed a significant increase in ATP...
20 February 2017 3,553 7 View
I am currently looking into reviving T-cells or B-cells from frozen human tissue. Is it feasible? If yes, is there a specific way on how to freeze the tissue in the first place to get the best...
11 November 2016 1,434 6 View
I am currently looking for a murine NK cell line to use, with no avail. Is there anyone that may have one to provide or point to the direction of getting one? Thank you
03 November 2016 3,691 3 View
Basically, we have unfixed aortas that we want to obtain cryosections from. Aortas and hearts are unfixed, and I was wondering if I can immerse them in OCT at room temperature and then store them...
20 April 2016 3,272 4 View
