I have been trying the multiplexing protocol, for some trials on mouse tissues samples, but I still struggle with the secondary antibodies. Which ones would you recommend?
Hi Almudena, I would recommend secondary's from Jackson labs. They have been immunodepleted.
https://www.jacksonimmuno.com/
I need to find a good antibody for T memory cells for IHC, and was wondering if anyone had found a suitable candidate for this.
06 July 2018 1,738 1 View
I am working on a multiplex staining with a rat antibody, for which I always block before incubating with the antibody. My question is, could it give me any unspecific signal on the fluorescent...
08 September 2017 544 2 View
I need to do some H&E staining on OCT samples, I have done some before on paraffin sections, so if possible to do, I guess it should be similar. Thank you
31 December 2016 4,805 6 View
I am trying out the multiplexing kit, and I am finding some trouble with the general protocol, anyone has done this and has some insights?
11 December 2016 1,649 2 View
I am trying a multiplexing IHC, and we don't have any microwave stronger than 800W, and I am not sure if this could be an issue. Also, is it possible to do any of the steps Overnight? Thanks
11 December 2016 6,697 6 View
I want to do a double staining on some IHC samples, can I use 2 different Peroxidase detection systems as AEC and DAB together? Which mounting media should I use (if the staining is possible)?
10 November 2016 656 5 View
I have always used the Blades low profile Feather Disposable Microtome Blade S35 for sectioning and cryosectioning but it seems Thermo has also this Blades low profile MX35 Ultra, has anyone used...
09 October 2016 3,895 5 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...
25 February 2021 7,603 3 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View