Almudena Ines Santos

8 Questions 8 Answers 0 Followers

Questions related from Almudena Ines Santos

Any good antibody to detect T memory cells for IHC?

I need to find a good antibody for T memory cells for IHC, and was wondering if anyone had found a suitable candidate for this.

07 July 2018 1,831 1 View

Multiplex, on mouse tissues. Serum block interference?

I am working on a multiplex staining with a rat antibody, for which I always block before incubating with the antibody. My question is, could it give me any unspecific signal on the fluorescent...

09 September 2017 603 2 View

Multiplex, on mouse tissues. Secondary antibodies

I have been trying the multiplexing protocol, for some trials on mouse tissues samples, but I still struggle with the secondary antibodies. Which ones would you recommend?

06 June 2017 467 1 View

Does someone have an H&E protocol for frozen OCT samples?

I need to do some H&E staining on OCT samples, I have done some before on paraffin sections, so if possible to do, I guess it should be similar. Thank you

01 January 2017 4,998 6 View

For a multiplexing IHC staining is there any step which I can do O/N? Is the 1000W microwave that important?

I am trying a multiplexing IHC, and we don't have any microwave stronger than 800W, and I am not sure if this could be an issue. Also, is it possible to do any of the steps Overnight? Thanks

12 December 2016 6,774 6 View

Does anyone have an optimised protocol for IHC multiplexing?

I am trying out the multiplexing kit, and I am finding some trouble with the general protocol, anyone has done this and has some insights?

12 December 2016 1,739 2 View

Can you use AEC and DAB for the same section in IHC?

I want to do a double staining on some IHC samples, can I use 2 different Peroxidase detection systems as AEC and DAB together? Which mounting media should I use (if the staining is possible)?

11 November 2016 729 5 View

Feather Disposable Microtome Blade S35 or Blades low profile MX35 Ultra Thermo Scientific?

I have always used the Blades low profile Feather Disposable Microtome Blade S35 for sectioning and cryosectioning but it seems Thermo has also this Blades low profile MX35 Ultra, has anyone used...

10 October 2016 4,012 5 View