Any good antibody to detect T memory cells for IHC?

I need to find a good antibody for T memory cells for IHC, and was wondering if anyone had found a suitable candidate for this.

06 July 2018 1,738 1 View

Multiplex, on mouse tissues. Serum block interference?

I am working on a multiplex staining with a rat antibody, for which I always block before incubating with the antibody. My question is, could it give me any unspecific signal on the fluorescent...

08 September 2017 544 2 View

Multiplex, on mouse tissues. Secondary antibodies

I have been trying the multiplexing protocol, for some trials on mouse tissues samples, but I still struggle with the secondary antibodies. Which ones would you recommend?

05 June 2017 386 1 View

Does someone have an H&E protocol for frozen OCT samples?

I need to do some H&E staining on OCT samples, I have done some before on paraffin sections, so if possible to do, I guess it should be similar. Thank you

31 December 2016 4,805 6 View

Does anyone have an optimised protocol for IHC multiplexing?

I am trying out the multiplexing kit, and I am finding some trouble with the general protocol, anyone has done this and has some insights?

11 December 2016 1,649 2 View

For a multiplexing IHC staining is there any step which I can do O/N? Is the 1000W microwave that important?

I am trying a multiplexing IHC, and we don't have any microwave stronger than 800W, and I am not sure if this could be an issue. Also, is it possible to do any of the steps Overnight? Thanks

11 December 2016 6,697 6 View

Can you use AEC and DAB for the same section in IHC?

I want to do a double staining on some IHC samples, can I use 2 different Peroxidase detection systems as AEC and DAB together? Which mounting media should I use (if the staining is possible)?

10 November 2016 656 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View