Does anyone have an optimised protocol for IHC multiplexing?

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I need to find a good antibody for T memory cells for IHC, and was wondering if anyone had found a suitable candidate for this.

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Multiplex, on mouse tissues. Serum block interference?

I am working on a multiplex staining with a rat antibody, for which I always block before incubating with the antibody. My question is, could it give me any unspecific signal on the fluorescent...

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Multiplex, on mouse tissues. Secondary antibodies

I have been trying the multiplexing protocol, for some trials on mouse tissues samples, but I still struggle with the secondary antibodies. Which ones would you recommend?

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Does someone have an H&E protocol for frozen OCT samples?

I need to do some H&E staining on OCT samples, I have done some before on paraffin sections, so if possible to do, I guess it should be similar. Thank you

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For a multiplexing IHC staining is there any step which I can do O/N? Is the 1000W microwave that important?

I am trying a multiplexing IHC, and we don't have any microwave stronger than 800W, and I am not sure if this could be an issue. Also, is it possible to do any of the steps Overnight? Thanks

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Can you use AEC and DAB for the same section in IHC?

I want to do a double staining on some IHC samples, can I use 2 different Peroxidase detection systems as AEC and DAB together? Which mounting media should I use (if the staining is possible)?

10 November 2016 656 5 View

Feather Disposable Microtome Blade S35 or Blades low profile MX35 Ultra Thermo Scientific?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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fatal error

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fatal error

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

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Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

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How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

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