Does anyone have an optimised protocol for IHC multiplexing?

More Almudena Ines Santos's questions See All

Any good antibody to detect T memory cells for IHC?

I need to find a good antibody for T memory cells for IHC, and was wondering if anyone had found a suitable candidate for this.

06 July 2018 1,834 1 View

Multiplex, on mouse tissues. Serum block interference?

I am working on a multiplex staining with a rat antibody, for which I always block before incubating with the antibody. My question is, could it give me any unspecific signal on the fluorescent...

08 September 2017 605 2 View

Multiplex, on mouse tissues. Secondary antibodies

I have been trying the multiplexing protocol, for some trials on mouse tissues samples, but I still struggle with the secondary antibodies. Which ones would you recommend?

05 June 2017 471 1 View

Does someone have an H&E protocol for frozen OCT samples?

I need to do some H&E staining on OCT samples, I have done some before on paraffin sections, so if possible to do, I guess it should be similar. Thank you

31 December 2016 5,000 6 View

For a multiplexing IHC staining is there any step which I can do O/N? Is the 1000W microwave that important?

I am trying a multiplexing IHC, and we don't have any microwave stronger than 800W, and I am not sure if this could be an issue. Also, is it possible to do any of the steps Overnight? Thanks

11 December 2016 6,778 6 View

Can you use AEC and DAB for the same section in IHC?

I want to do a double staining on some IHC samples, can I use 2 different Peroxidase detection systems as AEC and DAB together? Which mounting media should I use (if the staining is possible)?

10 November 2016 732 5 View

Feather Disposable Microtome Blade S35 or Blades low profile MX35 Ultra Thermo Scientific?

I have always used the Blades low profile Feather Disposable Microtome Blade S35 for sectioning and cryosectioning but it seems Thermo has also this Blades low profile MX35 Ultra, has anyone used...

09 October 2016 4,019 5 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View