Can I use tryptone or tryptone peptone in place of trypticase to grow my microbe culture?

The ATCC media preparation mentions trypticase and I am not able to find trypticase from any company like HiMedia, Merck or Sigma

26 October 2020 1,822 6 View

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04 July 2020 9,111 0 View

Can we simulate blockchain network in NS#?

I am new to ns3 and I want to know if it is possible to simulate blockchain network on NS3 and if yes, can anyone share some links to tutorials that would be helpful for this. It would be helpful...

08 March 2020 7,384 1 View

Which type of batteries can be used to store power generated by C4MJ solar cell?

I want to use Concentrating solar cell developed by Spectro lab, USA and wish sole guidelines for the same. Is it possible to store the power generated by these cells be stored by any type of...

06 January 2020 8,313 3 View

Why I am getting two bands on agarose gel after pcr amplification of a mini prep plasmid having gene of interest with gene specific primers?

I have a purified plasmid having the insert of 655 bp. when i am pcr amplifying it with gene specific primer ideally i should get single amplified band of size 655 bp but after running it on gel i...

26 July 2019 1,762 7 View

How to add randomness to a channel using Matlab simulations?

I have to simulate multipath fading channels and add randomness to it.

21 September 2018 6,377 2 View

No amplification after PCR?

I successfully amplified my samples using Primer set 1 but when I'm amplifying the same samples using different Primer set, the genomic DNA is still stuck in the Wells and only ladder resolves. My...

09 July 2018 9,186 13 View

Can someone give me suggestion over following choice experiment as to how I can place the variables in a right way?

Please suggest me how can I better place the variables in the following choice experiment. CE1. Please look at the following scenario and select the level of self-driving car you would consider...

03 April 2018 1,864 2 View

How to get rid of bubbles in plastic film?

Hi, What could be the reasons that a film prepared by casting a film forming solution on petri plate gets bubbles into it? How can it be prevented? Thanks, Ashish

08 February 2018 3,804 4 View

Does any one know best kit for RNA extraction form human blood cell for gene exression using real time ?

I am setting up experiment to isolate RNA from human blood cell and use further for gene expression study.

03 November 2017 5,796 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View